FastQCFastQC Report
Wed 25 May 2016
SRR1781260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284030
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8444729.731718480442204No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG12890.4538253001443509No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9900.34855473013414073No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6640.2337781220293631No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.14364679787346407No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3600.12674717459423301No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG200.00694232352.508321
TTACGAC200.00694232352.508323
CTTAGGG200.00694232352.508323
GCGATAT200.006947159352.49907720
GCCGTAT2750.047.0900854
TAAGTTA305.8723975E-446.66584829
TTTGCCC305.8723975E-446.66584835
CGTATCA2850.045.43779856
TCTCGGT2850.045.43779843
ATCATTA2850.045.43779859
TATCATT2800.044.99920758
CATTAAA2750.044.5446761
TCATTAA2900.043.44751460
CCGTATC2900.043.44751455
AGATCTC3050.042.45826740
TAGATCT3050.042.45826739
GTATCAT3100.041.7734657
CGCCGTA2950.041.52469353
TCGGTGG3400.040.1463545
GTTCTTA350.00125319640.006342