FastQCFastQC Report
Wed 25 May 2016
SRR1781260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284030
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT4111514.475583565116363No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG74722.630708023800303No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT53751.8924057317888956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC40591.4290743935499772No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT34121.2012815547653417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23820.8386438052318417No Hit
CATAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT11890.4186177516459529No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11650.41016794000633733No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6740.2372988768792029No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT6520.22955321620955535Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT6420.2260324613597155No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT5050.1777981199169102No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4900.17251698764215048No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT4020.14153434496356018No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT3860.1359011372038165No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3320.11688906101468155No Hit
TCTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG3240.1140724571348097No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC150.002219694270.0276641
AGGGGGG48800.063.8951770
CTTAAAC11600.060.638471
CCGAATT308.344356E-658.3563847
TAACGAA308.3618015E-658.33582738
TTAAACA12450.058.1850172
TAAACAC16550.058.149353
TTGATAA1400.057.49233629
TCTTTAT2200.057.265091
GACTAGC4050.057.02943426
AACACAT16500.056.8409045
AAACACA18550.056.596234
ACTTATG252.3853804E-456.01225750
GGATAGT252.389536E-455.9925358
AGAATTG750.055.99253525
CTAGCTA3700.055.8033728
GAGACTA4150.055.6552324
AGACTAG4250.055.16911325
CATATAC9100.054.992671
AGCTAAG3900.054.73629430
Produced by FastQC (version 0.11.5)