FastQCFastQC Report
Wed 25 May 2016
SRR1781259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246236
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7116528.901135496028203No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG10950.4446953329326337No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8360.3395116879741386No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5030.20427557302750207No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.12792605467925078No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3000.12183433778976266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002222822770.054
TTGTGCG352.9662988E-760.02
ATGTCAA200.006945582652.532
TGGTTGC200.006945582652.543
ACGTCAT200.006945582652.517
CTTTATA200.006945582652.52
ATCGGAT200.006945582652.570
ACGCTTT305.870356E-446.66666413
GCCGTAT2500.044.854
CGTATCA2750.044.54545256
TCTCGGT2750.044.54545243
CCGTATC2550.043.9215755
GTATCAT2850.042.98245657
TAGATCT3050.042.45901539
TCATTAA3000.042.060
ATCATTA2950.041.52542559
GCGTGTG1100.041.3636368
AGATCTC3050.041.31147440
CATTAAA3250.040.92307761
GTAGATC3250.040.92307738
Produced by FastQC (version 0.11.5)