FastQCFastQC Report
Wed 25 May 2016
SRR1781259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246236
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2748711.162868142757356No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG48061.951786091391998No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT29361.1923520525024773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC28601.1614873535957373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21840.8869539791094722No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT17340.7042024724248283No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT12200.4954596403450348No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT11600.4710927727870823No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6380.2591010250328953No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT5960.24204421774232848No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT5630.2286424405854546No Hit
CATAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5040.2046816874868013No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT3550.14417063305121913No Hit
CTTATACACATCTCCGCGCCCACGAGACATTGCCGGATCTCGTATGCCGT3480.14132783183612468No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA3150.12792605467925078No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCG3150.12792605467925078No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT3090.12548936792345555No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACG3040.12345879562695951No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT2960.12020987995256584No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2910.11817930765606979No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2490.10112250036550302RNA PCR Primer, Index 30 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG207.864043E-570.09357543
ACGTAAT750.065.4206747
GACGTAA650.064.7017646
AGGGGGG28600.062.6391670
ATATCGA401.0650183E-861.2322337
ATCGAAT352.9680814E-759.99477439
TAAACAC15750.059.3161163
TAAGCCG3500.059.0788743
CTTAAAC11250.058.782941
AAACACA17950.058.6737754
AACACAT16300.058.6026235
TACCGTC308.294655E-658.41131645
TTAAACA11450.057.7561652
GCCGAAA553.45608E-1157.34928546
CGTAAGA800.056.91629441
TCGTAAG3950.056.71544640
CTCGTAA3950.056.70392639
CGTAATA1800.056.46426848
CATAAAC4900.056.41221
GTCATAT2050.056.3709149
Produced by FastQC (version 0.11.5)