FastQCFastQC Report
Wed 25 May 2016
SRR1781258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167868
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10729563.91629137179212No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG33521.9968070150356232No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11680.6957847832821026No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10000.5957061500702934No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4890.29130030738437346No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.2692591798317726No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3170.188838849572283No Hit
TCTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGT3100.18466890652179094No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3090.18407320037172062No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.1626277789691901No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2170.12926823456525366No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.11497128696356662No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.11497128696356662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA252.8304294E-670.038
GAGTGTA150.002221586670.034
GATATCG150.002221586670.041
ATGACGC207.908939E-570.012
GTGTAGG301.0189615E-770.036
TATGACG150.002221586670.011
AGAACTT150.002221586670.054
GTGAGGA150.002221586670.01
CTTGGGG401.3460522E-1070.01
ACATATG207.908939E-570.08
AGTGTAG252.8304294E-670.035
TGTAGGT207.908939E-570.037
ATATGAC150.002221586670.010
CTTGTGC353.6870915E-970.01
GTAGGTC150.002221586670.038
TGTGGGC301.0189615E-770.03
ACGATTA150.002221586670.024
CCCCTGC150.002221586670.021
GCCGTAA150.002221586670.054
TTGTGGG353.6870915E-970.02
Produced by FastQC (version 0.11.5)