FastQCFastQC Report
Wed 25 May 2016
SRR1781258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167868
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT5273931.416946648557197No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG92385.50313341434937No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT68874.1026282555341105No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57953.45211713965735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC53553.190006433626421No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT36792.191602926108609No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT15650.932280124860009No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC13390.7976505349441227No Hit
CATAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT13250.7893106488431386No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT7630.4545237925036338No Hit
CTTATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT6390.38065622989491743No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6130.3651678699930898Illumina PCR Primer Index 10 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5970.3556365715919651Illumina PCR Primer Index 10 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4760.2835561274334596No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTAAGCCGT4400.26211070603092906No Hit
TCTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG4200.2501965830295232No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT4200.2501965830295232No Hit
CTCTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4200.2501965830295232No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG3860.22994257392713324No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3790.22577263087664115No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACG3690.2198155693759382No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATAATCTCGTATGCCGT3500.20849715252460266No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3460.2061143279243215Illumina PCR Primer Index 10 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG3450.2055186217742512No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGA3390.20194438487382943No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGAATATCTCGTATGCCGT3300.19658302952319678No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT3120.18586031882193152No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3080.18347749422165033No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTAAGCCGT2830.16858484046989303No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT2800.16679772201968213No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAG2730.1626277789691901No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAAGATTATCTCGTATGCCGT2700.1608406605189792No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2680.1596492482188386Illumina PCR Primer Index 10 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2390.14237376986680012No Hit
ATACCCATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT2330.13879953296637834Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCACCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2150.12807682226511308No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT2110.12569399766483189Illumina PCR Primer Index 10 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGA2070.12331117306455072No Hit
CTTATACACATCTCCGAGCCAACGAGACAGTAGATTATCTCGTATGCCGT2040.12152405461433984No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGAATGCCGT2010.11973693616412896No Hit
CTTATACACATCTCCGAGACCACGAGACAGTAGATTATCTCGTATGCCGT1870.11139705006314486No Hit
CTTAAACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT1790.10663140086258249No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGCTTATCTCGTATGCCGT1690.10067433936187958TruSeq Adapter, Index 10 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG150.002219224370.01877643
TATGACC150.002219224370.01877643
TGCCTTA150.002219224370.01877645
CCGTAAA150.002219224370.01877647
TATCCCG150.002219224370.01877643
GACGTAA150.002219224370.01877646
TAATCCG150.002219224370.01877643
ACCTCTT150.002219224370.01877647
AGACTTC252.825922E-670.01877648
TCCGAAT150.002219224370.01877646
AATCCGT207.898437E-570.0187744
ATGACCT207.898437E-570.0187744
TATGCCA401.3460522E-1070.0187743
CAGACTT207.898437E-570.0187747
CAGAAAT207.898437E-570.0187747
CGACTTA401.3460522E-1070.0187748
AAACCGA150.00222184969.9979211
TCCTTAT301.01917976E-769.997921
AACAAGT252.8309296E-669.9979237
AACCGAG500.069.9979212
Produced by FastQC (version 0.11.5)