FastQCFastQC Report
Wed 25 May 2016
SRR1781257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328490
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8888427.058357940881No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG14760.44932874668939693No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11030.33577886693658865No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6570.2000060884654023No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4650.1415568206033669No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4330.13181527595969436No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC150.0022210770.0191957
TTGTACG207.9213976E-569.997862
GCCGTAT2750.062.36173654
CGTATCA2850.058.94557656
CCGTATC2950.058.13382355
GTAGATC3150.053.33170738
CATATGG200.00694847752.4983989
GGCACTT200.00694847752.49839815
GTATCAT3150.052.23653457
TCTCGGT3350.050.14772843
CGCCGTA3450.049.70863353
TCGGTGG3750.048.53185745
TAGATCT3450.047.6797139
TATCATT3350.047.02781758
ATCATTA3350.047.02781759
CATTAAA3450.046.6794661
ATCTCGG3450.046.6652542
GGTACAA305.87412E-446.6652451
CGGTGGT3850.046.36222546
TGTAGAT3700.046.3499437
Produced by FastQC (version 0.11.5)