FastQCFastQC Report
Wed 25 May 2016
SRR1781257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328490
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4467913.601327285457701No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG76302.3227495509756766No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT55541.6907668422174191No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC51771.5759992693841516No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT29860.9090078845626961No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28190.8581691984535298No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC11530.351000030442327No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT11030.33577886693658865No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT9390.28585345063776674No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT5820.17717434320679473No Hit
CTTATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT5320.16195317970105635No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5180.1576912539194496RNA PCR Primer, Index 24 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4940.15038509543669518No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT4310.1312064294194648No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT3520.10715699108039818No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3310.10076410240798808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002211555870.09546
TAACGTA455.456968E-1269.9882838
ATCGTAA252.839297E-669.9882839
TAACCGA150.00222630769.9776211
ATAACCG150.00222630769.9776210
AGGGGGG52400.064.7025970
ACGTATG1800.062.22127540
AACGTAT453.8380676E-1062.21180739
ACGTAAG401.0641088E-861.24906540
CATATCG2250.060.65650636
CGTCTAA352.944762E-760.0722748
TCTTTAT2450.059.980821
ACGTCTA553.45608E-1157.35045647
CGAATGC2750.057.3242341
ATAGCAA2750.057.25441732
GCATATC2200.057.25441735
TCGAATG2700.057.03616740
ATATCGT2150.056.96720537
CACGTAT1600.056.86547539
TATCGTA2100.056.65717338
Produced by FastQC (version 0.11.5)