FastQCFastQC Report
Wed 25 May 2016
SRR1781256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283390
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9329032.91929849324253No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG17600.6210522601362081No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9420.3324041074138113No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7710.27206323441194113No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4700.16584918310455554No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3080.1086841455238364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3250.054.92113554
CCGTATC3300.054.08955
CGTATCA3450.052.75176256
TACGAAT200.0069427852.5074167
TAGGAGC200.0069427852.50741637
CGTTCTA200.00694762852.4981514
TTAAGGT200.00694762852.498154
CGCCGTA3550.052.2516853
ATCATTA3500.051.99816559
TATCATT3450.051.73730558
TCTCGGT3600.051.5259643
CATTAAA3700.050.13336661
GTATCAT3600.049.58158557
TAGATCT3600.049.58158539
ATCTCGG3700.049.18745442
TCATTAA3650.048.90238660
AGATCTC3900.048.45982740
GTAGATC3800.047.89304738
CGCGTCT305.8677787E-446.6732527
TGGTCGC3950.046.0743249
Produced by FastQC (version 0.11.5)