FastQCFastQC Report
Wed 25 May 2016
SRR1781256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283390
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4429915.631814813507885No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG84012.964465930343343No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT68692.423868167542962No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC42861.5124034016726067No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT34651.2226966371431596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30441.0741381135537598No Hit
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT13410.4731994777515085No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC12630.44567557076819925No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT8850.3122904830798546No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT6590.2325417269487279TruSeq Adapter, Index 12 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5560.19619605490666572TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT5350.18878577225731324No Hit
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT5270.1859628074385123No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT4600.16232047708105438No Hit
TCTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG4110.14502981756589858No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT3790.1337379582906948No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3620.1277391580507428No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT3400.11997600479904018No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3270.11538868696848867No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT3030.10691979251208582No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2930.10339108648858464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATC150.002223725469.9955940
TGTAGCG207.927726E-569.983232
ATTTCCG150.002225282869.9832319
AGGGGGG52450.065.0463970
ATCGTAA453.8380676E-1062.21830439
CTTAAAC15650.061.039691
TAAACAC20700.060.6859473
AACACAT21500.060.5436335
TAAGCCG3300.060.50412843
TTAAACA16100.059.768292
GTAAGCC3400.059.7548542
AAGCCGT3350.059.6010844
AAACACA23400.059.2165834
CGTAAGC3350.058.54510541
GACGTAT308.314599E-658.39148746
TATGACG3900.056.5848343
TATCGTA2300.056.30079738
CATAAAC5600.056.2365341
CATATAC9000.055.2089961
TCGTAAG3250.054.91961740
Produced by FastQC (version 0.11.5)