FastQCFastQC Report
Wed 25 May 2016
SRR1781255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383463
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10278926.805454502781235No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG16940.4417636121346779No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9850.2568696327937767No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9330.24330900243309003No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5400.14082193066866946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC707.2759576E-1250.03
GCCGTAT3650.048.9041154
CGTATCA3950.046.9620256
CCGTATC3850.046.36363655
ATCATTA4100.042.68292659
TCTCGGT4200.042.50000443
TCATTAA4300.040.69767460
CGCCGTA4300.040.69767453
GTAGATC4400.040.56818438
TCGGTGG5100.040.49019645
ATCTCGG4350.040.2298842
GTATCAT4350.040.2298857
AGATCTC4350.039.42528540
GATCTCG4450.039.32584441
CGGTGGT5200.039.0384646
TGGTCGC4700.038.72340449
TAGATCT4600.038.0434839
TTGGGCG654.85883E-737.6923072
TGTAGAT5150.037.3786437
CATTAAA4700.037.23404361