FastQCFastQC Report
Wed 25 May 2016
SRR1781255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383463
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT4777012.457525237115446TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG81572.127193497156179TruSeq Adapter, Index 5 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT57071.488279182085364RNA PCR Primer, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC44161.151610455246008RNA PCR Primer, Index 5 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT32400.8449315840120168TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30600.7979909404557937No Hit
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT12410.32362965918484965TruSeq Adapter, Index 5 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC12250.3194571575354076RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT11150.29077120869549344No Hit
CATAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT10700.27903604780643765No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT7100.18515476069399134No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT6500.16950787950858362TruSeq Adapter, Index 5 (96% over 26bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC5460.14238661878721023TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT5310.1384748984908583No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT5170.13482395954759652No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT4420.11526535806583686No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT4290.11187520047566518No Hit
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3890.10144394635206004No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT3840.1001400395866094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG50950.062.29260370
CACGTAA1100.060.45713439
TAAACAC22250.059.7622533
CTTAAAC15900.057.6603851
AACACAT23300.057.3694735
AAACACA25400.057.1725734
TTAAACA16150.056.767812
CATAAAC7000.054.9880181
GCATGAA13400.053.28586656
CATGAAA14400.052.74531657
GACGAAT407.422004E-752.5502346
TCGTATC200.00694376152.50909840
ATGACGA1150.051.7886344
AAGCCGT4400.051.75401344
CGTATGA5850.051.49549541
ATGAAAA16500.051.123458
CTGCATG9100.050.7979154
ATCTCGT104600.050.7824337
ATATACA11050.050.667692
TCGTATG107250.050.656840
Produced by FastQC (version 0.11.5)