FastQCFastQC Report
Wed 25 May 2016
SRR1781254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334859
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8179424.42640036552698No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG31740.9478616372861414No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10190.3043071860096339No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6600.1970978829895568No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4400.13139858865970452No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3750.11198743351679365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT6050.055.5371954
CGTATCA5900.055.16949556
GTAGATC6350.054.0157538
GTATCAT5900.053.3898357
TCATTAA5850.053.24786460
ATCATTA6050.053.2231459
CCGTATC6250.053.255
GTCTACT200.006947757752.52
TAGATCT6650.052.10526339
CGCCGTA6650.052.10526353
TATCATT6050.051.48760258
CATTAAA6450.050.4651261
TCTCGGT6850.050.07299443
ATCTCGG7050.049.64539342
AGATCTC7050.049.64539340
TGGTCGC7050.049.1489449
GTGGTCG7200.049.09721848
TTGTGCG506.1838364E-849.0000042
TGTAGAT7650.048.49673537
GATCTCG7450.047.9194641
Produced by FastQC (version 0.11.5)