FastQCFastQC Report
Wed 25 May 2016
SRR1781254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334859
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4195312.528556795546782No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG93752.799685837919841No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT65761.9638116341504932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54501.6275507004440675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC47061.405367632346749No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC13080.39061216810657623No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT10490.3132661806909774RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT9100.2717561720007526No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT8480.253240916325976No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC7680.22935026384239338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT6600.1970978829895568No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6030.18007579309500418No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTCGTCAATCTCGTATGCCGT5910.17649219522246676No Hit
CTTATACACATCTCCGAGACCACGAGACTTTCGTCAATCTCGTATGCCGT3950.1179600966376893No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT3670.10959836826843537No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3590.1072093030200771No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3520.10511887092776362No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3500.10452160461567406RNA PCR Primer, Index 48 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT207.921847E-569.9974637
AATCCGA150.00222516169.9870111
ATACGTC150.00222516169.9870129
ATTATAC16000.062.76961
AGGGGGG51200.062.67391670
ATTTCGT800.056.86444528
GCCGTAA509.713403E-1056.04819546
ACGTATG1250.056.00633240
CGTAAAT750.055.9896132
CACGTAT1200.055.41465839
GCCGTAT700.055.04733346
AATATAC1100.054.080871
AATCACG1300.053.844236
AATTTCG850.053.51947827
TGCCGTA1050.053.3792345
CCGTCTA1750.052.04474647
CGTCTAC1700.051.5148848
TATGCCG93600.050.67391243
TCGTAAA900.050.54617331
ATGCCGT93100.050.4192944
Produced by FastQC (version 0.11.5)