FastQCFastQC Report
Wed 25 May 2016
SRR1781253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236317
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6652428.15032350613794No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG23220.9825784856781357No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8540.36137899516327643No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5220.22088973709043358No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3770.15953147678753538No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3390.14345138098401722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG252.8340091E-670.042
TTGTGCG453.8380676E-1062.2222182
CGTATCA4600.059.34782456
GCCGTAT4700.058.82978454
TCATTAA4500.058.33333660
GATATCG308.359591E-658.33333641
TATCGGT308.359591E-658.33333643
CCGTATC4650.057.9569955
ATCATTA4600.057.82608459
GTATCAT4800.056.14583657
AACTGCG252.3871385E-455.9999967
TATCATT4700.055.8510658
CGCCGTA4900.055.71428753
CATTAAA4950.055.15151661
TCTCGGT4900.053.5714343
ACGGGTT200.006945238452.524
TGTGATC200.006945238452.512
ATATTGA200.006945238452.56
GCTTTGT200.006945238452.531
TGGTCGC5050.051.98019849
Produced by FastQC (version 0.11.5)