SRR1781253_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781253_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236317
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	33094	14.004070803200786	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	7075	2.9938599423655514	No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	6219	2.6316346263705106	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4115	1.7413051113546634	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	3834	1.6223970344918057	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	955	0.4041181971673642	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT	912	0.3859222992844357	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	772	0.3266798410609478	TruSeq Adapter, Index 2 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	554	0.2344308703986594	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT	550	0.23273822873513117	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	528	0.22342869958572592	No Hit
CTTATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT	427	0.18068949758163821	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT	423	0.17899685591811	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT	423	0.17899685591811	No Hit
CTTATACACATCTCCGAGACCACGAGACGAGCACGTATCTCGTATGCCGT	381	0.1612241184510636	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT	377	0.15953147678753538	No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT	369	0.15614619346047892	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGA	287	0.12144703935815027	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTAAGCCGT	283	0.11975439769462204	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	274	0.11594595395168353	No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT	272	0.11509963311991941	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT	270	0.1142533122881553	No Hit
CTTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT	268	0.11340699145639119	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	265	0.11213751020874502	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	256	0.1083290664658065	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATAC	1215	0.0	64.80776	1
AGGGGGG	3830	0.0	62.31698	70
ACGTAAG	30	8.354498E-6	58.339325	40
AAGCTTG	55	3.6379788E-11	57.29074	54
CGTAAGC	160	0.0	54.7047	41
TATCCGA	240	0.0	53.95246	11
AATGCCG	105	0.0	53.350105	43
ATCTTAT	210	0.0	53.32753	1
ACATATC	230	0.0	53.25507	8
TCGTAAG	165	0.0	53.03575	40
AATCCGT	20	0.006936604	52.516514	44
GTTAGTT	20	0.006936604	52.516514	58
TAAGCCG	160	0.0	52.516514	43
GACCCAT	20	0.006942414	52.505398	38
GGGGATA	20	0.006948228	52.49429	21
CTCCGAT	20	0.006948228	52.49429	12
GGTCACT	20	0.006948228	52.49429	22
TATGAAC	20	0.006948228	52.49429	4
CGTAACA	40	7.4644413E-7	52.49429	34
ACGACAC	20	0.006948228	52.49429	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content