FastQCFastQC Report
Wed 25 May 2016
SRR1781252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61392
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3084850.24758926244461No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG8821.4366692728694292No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5780.9414907479801929No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3990.6499218139171228No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2320.37789940057336463No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1510.24596038571800885No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.20686734427938494No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1210.19709408391972894No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.19220745373990095No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT860.1400833984884024No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850.13845452176179307No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.12053687776909043No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720.11727912431587179No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG620.10099035704977848No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA150.002214852370.015
TAAACAA252.8090453E-670.05
GGATCAT150.002214852370.057
GATCATT150.002214852370.058
TAATAAA150.002214852370.02
TTGTGCG500.070.02
GGGTGTA150.002214852370.034
CGCGTGT150.002214852370.07
TGTGCGC500.070.03
CTTGTGC501.2732926E-1162.9999961
TGGCGCT850.061.76470613
TGCGCGT352.929446E-760.05
GTCTGGC352.929446E-760.010
TTGGGCG308.286319E-658.3333322
TTGTGGG308.286319E-658.3333322
GCGTCGG252.3731786E-455.9999968
GGTGTAG252.3731786E-455.99999635
CGCTGGC452.647539E-854.44444316
CGTCTGG452.647539E-854.4444439
TGCCGGC653.6379788E-1253.84615319
Produced by FastQC (version 0.11.5)