##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61392 Sequences flagged as poor quality 0 Sequence length 76 %GC 88 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.475664581704457 32.0 27.0 32.0 13.0 32.0 2 26.932140995569455 32.0 27.0 32.0 13.0 32.0 3 26.743223872817307 32.0 27.0 32.0 13.0 32.0 4 26.107750195465208 32.0 22.0 32.0 8.0 32.0 5 26.869510685431326 32.0 27.0 32.0 13.0 32.0 6 30.31150638519677 37.0 27.0 37.0 8.0 37.0 7 29.610714751107636 37.0 22.0 37.0 8.0 37.0 8 30.61615519937451 37.0 27.0 37.0 8.0 37.0 9 30.435382460255408 37.0 27.0 37.0 13.0 37.0 10-11 30.213285118582228 37.0 27.0 37.0 10.5 37.0 12-13 29.91816523325515 37.0 24.5 37.0 10.5 37.0 14-15 31.625252475892623 37.0 29.5 37.0 13.0 37.0 16-17 31.748248957518896 37.0 29.5 37.0 13.0 37.0 18-19 30.647397054990876 37.0 29.5 37.0 13.0 37.0 20-21 31.56643373729476 37.0 29.5 37.0 13.0 37.0 22-23 31.6429420771436 37.0 29.5 37.0 13.0 37.0 24-25 30.85810040396143 37.0 29.5 37.0 13.0 37.0 26-27 31.795861024237684 37.0 32.0 37.0 13.0 37.0 28-29 30.79224491790461 37.0 29.5 37.0 13.0 37.0 30-31 31.057173573103988 37.0 32.0 37.0 13.0 37.0 32-33 31.287415298410217 37.0 32.0 37.0 13.0 37.0 34-35 31.29372719572583 37.0 32.0 37.0 13.0 37.0 36-37 32.32769741985926 37.0 32.0 37.0 13.0 37.0 38-39 32.300112392494135 37.0 32.0 37.0 13.0 37.0 40-41 31.39801602814699 37.0 29.5 37.0 13.0 37.0 42-43 31.391394644253324 37.0 32.0 37.0 13.0 37.0 44-45 30.98717259577795 37.0 29.5 37.0 13.0 37.0 46-47 31.61946996351316 37.0 29.5 37.0 13.0 37.0 48-49 32.42357310398749 37.0 32.0 37.0 13.0 37.0 50-51 32.95663930153766 37.0 34.5 37.0 13.0 37.0 52-53 31.660933020589 37.0 32.0 37.0 13.0 37.0 54-55 31.938794956997654 37.0 32.0 37.0 13.0 37.0 56-57 31.65082584050039 37.0 29.5 37.0 13.0 37.0 58-59 30.92670054730258 37.0 29.5 37.0 13.0 37.0 60-61 30.448413474068282 37.0 27.0 37.0 13.0 37.0 62-63 29.91611284857962 37.0 24.5 37.0 8.0 37.0 64-65 29.529629267657022 37.0 24.5 37.0 8.0 37.0 66-67 29.652593171748762 37.0 22.0 37.0 8.0 37.0 68-69 29.885595843106593 37.0 27.0 37.0 10.5 37.0 70-71 29.926317761271825 37.0 24.5 37.0 13.0 37.0 72-73 30.730559356267918 37.0 27.0 37.0 13.0 37.0 74-75 31.680829749804538 37.0 32.0 37.0 13.0 37.0 76 32.55793914516549 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.4059377378326623 11101 2 0.9748925504118269 11101 3 -0.4232858655085785 11101 4 0.5869409607342568 11101 5 -0.3108810536577913 11101 6 -0.6901219853606904 11101 7 1.6420620391088185 11101 8 -2.302506909194175 11101 9 -1.8438831408583134 11101 10-11 -1.8365206533073888 11101 12-13 -1.251639530807033 11101 14-15 -0.11850536895212826 11101 16-17 -3.795288942372583 11101 18-19 -0.5382367725381556 11101 20-21 -0.6702470949009758 11101 22-23 -1.3420554252217016 11101 24-25 -1.430889171768328 11101 26-27 -2.029929966637191 11101 28-29 -0.3091519748397751 11101 30-31 -1.0430893350456714 11101 32-33 -0.40017145680418054 11101 34-35 0.4785855337500422 11101 36-37 -1.2542183267157583 11101 38-39 -0.20210479776563517 11101 40-41 -1.941610624087975 11101 42-43 0.5281780867767054 11101 44-45 -1.539899564347472 11101 46-47 -1.4309442231394307 11101 48-49 -0.4546122005448652 11101 50-51 -0.9838270882674323 11101 52-53 0.4333290088132138 11101 54-55 -0.7277414062197671 11101 56-57 0.8057776709004614 11101 58-59 -1.1227184354670285 11101 60-61 -1.4744442962336564 11101 62-63 -1.6713832410438698 11101 64-65 -1.0854131081432996 11101 66-67 -1.4566630124871303 11101 68-69 0.4093613500057387 11101 70-71 -2.0098058429430097 11101 72-73 -0.26411979864727186 11101 74-75 -4.212123227510947 11101 76 -5.343892283002138 11102 1 0.5011758330707572 11102 2 0.85584493136421 11102 3 -1.2280477702704857 11102 4 -2.020201896408601 11102 5 -2.603738196514932 11102 6 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28-29 -0.38248530817310566 21611 30-31 0.5835773316209973 21611 32-33 0.6998285431958244 21611 34-35 -0.2514144662499582 21611 36-37 -1.5642183267157606 21611 38-39 0.5978952022343691 21611 40-41 -0.2616106240879752 21611 42-43 0.9781780867767083 21611 44-45 0.40343376898586314 21611 46-47 -0.7909442231394301 21611 48-49 -0.4079455338781983 21611 50-51 -1.5704937549341018 21611 52-53 0.5233290088132172 21611 54-55 1.1822585937802295 21611 56-57 -1.4742223290995398 21611 58-59 -0.4227184354670328 21611 60-61 -1.4544442962336532 21611 62-63 0.6019500922894636 21611 64-65 0.18458689185670352 21611 66-67 -1.2866630124871286 21611 68-69 -1.173971983327597 21611 70-71 -1.9031391762763477 21611 72-73 -0.4907864653139349 21611 74-75 -0.12545656084428103 21611 76 -0.3172256163354703 21612 1 1.3385618513078406 21612 2 0.35432517452530377 21612 3 1.5774233543495768 21612 4 2.980557982010854 21612 5 2.608267882512422 21612 6 2.170870922440727 21612 7 1.7271684220875407 21612 8 2.8989115305221382 21612 9 1.902216149921827 21612 10-11 2.019862325416014 21612 12-13 2.369991674866725 21612 14-15 1.9786577516152484 21612 16-17 0.6813068023082636 21612 18-19 1.3674369863270925 21612 20-21 0.8492564511983147 21612 22-23 0.44730627690595526 21612 24-25 1.9439335232671269 21612 26-27 1.814396274497561 21612 28-29 1.503259372677963 21612 30-31 0.9565560550252492 21612 32-33 0.6764242878766709 21612 34-35 1.8562451082181255 21612 36-37 -0.05209066714129307 21612 38-39 0.9074696703194789 21612 40-41 1.4881766099545821 21612 42-43 1.3409440442235123 21612 44-45 1.4232210030284165 21612 46-47 1.5403323726052491 21612 48-49 2.625884253355842 21612 50-51 0.9431232663424964 21612 52-53 2.2663077322174736 21612 54-55 0.8743862533546967 21612 56-57 0.9621606496238613 21612 58-59 -0.5886758822755418 21612 60-61 1.3595982569578346 21612 62-63 1.8832266880341422 21612 64-65 0.6433102961120198 21612 66-67 1.8061029449596795 21612 68-69 1.6483684422043225 21612 70-71 -0.1516498145742169 21612 72-73 0.5319794921328693 21612 74-75 -0.5316267736102382 21612 76 1.4078807666432525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 29.0 13 70.0 14 162.0 15 333.0 16 557.0 17 849.0 18 1163.0 19 1283.0 20 1328.0 21 1357.0 22 1325.0 23 1528.0 24 1584.0 25 1649.0 26 1777.0 27 1922.0 28 2004.0 29 2287.0 30 2658.0 31 3022.0 32 3719.0 33 4702.0 34 6189.0 35 8482.0 36 11407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 76.50345321866041 4.1699244201198855 3.2772999739379722 16.04932238728173 2 74.13343758144384 4.881743549648163 13.149921813917123 7.834897054990879 3 71.72921553296845 4.871970289288507 15.290265832681783 8.108548345061246 4 75.83561376075059 7.768113109199895 6.108287724784988 10.28798540526453 5 74.85665884805837 7.312027625749283 12.185626791764399 5.6456867344279384 6 80.87210059942663 10.017591868647381 3.1860828772478498 5.9242246546781345 7 76.67448527495439 6.308639562157936 3.215402658326818 13.801472504560856 8 79.26114151681001 10.621905134219443 3.4499609069585615 6.666992442011989 9 73.47862913734689 4.147120145947355 2.871709669012249 19.502541047693512 10-11 78.31883633046651 7.114933541829554 7.224068282512379 7.342161845191556 12-13 75.91787203544436 4.860568152202242 8.142754756320041 11.07880505603336 14-15 81.36320693249934 5.538180870471723 3.937809486578056 9.160802710450874 16-17 80.52840761011207 4.595061245764921 3.447517591868647 11.429013552254364 18-19 76.80153765962991 4.52339066979411 6.377052384675527 12.298019285900443 20-21 80.6570888715142 4.252182694813657 4.208203023195205 10.882525410476935 22-23 80.23928199113891 4.089295022152723 3.3286095908261664 12.3428133958822 24-25 79.21960516028147 5.95680218921032 3.164093041438624 11.659499609069586 26-27 81.71422986708366 6.345289288506646 3.5656111545478235 8.374869689861871 28-29 79.59994787594475 6.91865389627313 5.5894904873599165 7.891907740422205 30-31 81.06430805316653 6.073266875162887 4.472081052905916 8.39034401876466 32-33 79.3833072713057 4.953414125618973 4.516060724524368 11.14721787855095 34-35 79.71641256189731 6.308639562157936 5.68966640604639 8.285281469898358 36-37 81.12035053712647 4.154483926927996 6.8380801902543515 7.887085345691179 38-39 81.12294761532446 4.187842064112588 7.181717487620537 7.507492832942403 40-41 81.21823690383113 6.250814438363304 4.954228563982277 7.5767200938233 42-43 79.432173573104 5.9739053948397185 6.710157675267135 7.883763356789158 44-45 78.52244592129267 4.685463904091739 7.28189340630701 9.510196768308575 46-47 80.22380766223613 4.46230779254626 5.244168621318739 10.06971592389888 48-49 81.04639040917382 4.294533489705499 6.731333072713057 7.927743028407611 50-51 82.79254626009903 4.348286421683607 5.198560072973677 7.660607245243679 52-53 78.87998436278343 4.833691686213188 6.760652853792025 9.525671097211363 54-55 80.71002736512901 4.478596559812353 4.762835548605682 10.048540526452959 56-57 80.62858352879854 4.774237685691947 4.459050039093041 10.13812874641647 58-59 79.09336721396924 6.838024498305968 6.368093562679176 7.7005147250456085 60-61 78.37584701589783 5.303622621839979 6.716673182173573 9.60385718008861 62-63 78.45775439396654 7.353683764721214 6.28023651675327 7.908325324558975 64-65 77.50358352879854 7.902495439145166 7.263975762314308 7.329945269741986 66-67 77.69253322908523 10.334408391972895 4.388193901485535 7.584864477456346 68-69 77.47182043262966 10.850762314308053 4.275801407349491 7.401615845712796 70-71 77.64692468074016 10.761988532707845 4.288017982799062 7.303068803752932 72-73 79.26765702371644 9.479248110502997 3.9060463904091733 7.347048475371384 74-75 80.53003648683867 7.839783685170707 4.230192859004431 7.3999869689861875 76 82.81697941099817 5.536551993745113 4.381678394579098 7.264790200677612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 2.5 12 3.0 13 4.0 14 4.0 15 3.0 16 4.0 17 5.0 18 4.0 19 3.5 20 6.0 21 8.0 22 8.0 23 8.5 24 8.0 25 7.0 26 14.0 27 23.0 28 36.0 29 47.0 30 42.0 31 54.0 32 64.5 33 58.0 34 74.0 35 81.5 36 93.0 37 113.0 38 135.5 39 167.5 40 205.0 41 390.5 42 548.0 43 661.0 44 631.5 45 351.5 46 214.0 47 210.5 48 200.0 49 197.0 50 201.0 51 193.5 52 202.5 53 240.0 54 261.0 55 325.0 56 407.5 57 477.5 58 529.0 59 559.0 60 598.5 61 679.5 62 751.0 63 750.5 64 729.5 65 700.0 66 722.0 67 753.0 68 755.5 69 752.5 70 742.5 71 738.0 72 706.0 73 627.0 74 638.5 75 697.0 76 662.0 77 567.0 78 462.0 79 417.0 80 417.5 81 409.5 82 368.5 83 336.0 84 327.5 85 301.5 86 294.5 87 305.0 88 346.0 89 398.0 90 437.0 91 507.0 92 549.0 93 628.0 94 813.5 95 1086.0 96 1252.0 97 1619.5 98 2814.0 99 18128.5 100 32616.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.144383633046651E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0016288767266093302 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.13161323951003 #Duplication Level Percentage of deduplicated Percentage of total 1 89.31750741839762 31.378681261402136 2 6.110905044510385 4.293719051342195 3 2.327522255192878 2.453088350273651 4 0.8531157270029673 1.198853270784467 5 0.3384643916913947 0.5945400052124055 6 0.1437314540059347 0.3029710711493354 7 0.13909495548961426 0.3420641125879593 8 0.12054896142433234 0.3388063591347407 9 0.06954747774480713 0.21989835809225958 >10 0.49146884272997027 3.3180218921032054 >50 0.04636498516320475 1.0636565024758926 >100 0.027818991097922848 1.8699504821475113 >500 0.00927299703264095 2.378160020849622 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.004636498516320475 50.24758926244461 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 30848 50.24758926244461 No Hit CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG 882 1.4366692728694292 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 578 0.9414907479801929 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 399 0.6499218139171228 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 232 0.37789940057336463 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 151 0.24596038571800885 No Hit GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 127 0.20686734427938494 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 121 0.19709408391972894 No Hit GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 118 0.19220745373990095 No Hit TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT 86 0.1400833984884024 No Hit GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 85 0.13845452176179307 No Hit GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 74 0.12053687776909043 No Hit TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 72 0.11727912431587179 No Hit GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG 62 0.10099035704977848 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0048866301798279905 0.0 4 0.0 0.0 0.0 0.008144383633046651 0.0 5 0.0 0.0 0.0 0.026062027625749284 0.0 6 0.0 0.0 0.0 0.026062027625749284 0.0 7 0.0 0.0 0.0 0.026062027625749284 0.0 8 0.0 0.0 0.0 0.026062027625749284 0.0 9 0.0 0.0 0.0 0.034206411258795935 0.0 10 0.0 0.0 0.0 0.035835287985405265 0.0 11 0.0 0.0 0.0 0.040721918165233253 0.0 12 0.0 0.0 0.0 0.040721918165233253 0.0 13 0.0 0.0 0.0 0.04235079489184258 0.0 14 0.0 0.0 0.0 0.04235079489184258 0.0 15 0.0 0.0 0.0 0.04235079489184258 0.0 16 0.0 0.0 0.0 0.04235079489184258 0.0 17 0.0 0.0 0.0 0.04235079489184258 0.0 18 0.0 0.0 0.0 0.04723742507167058 0.0 19 0.0 0.0 0.0 0.057010685431326556 0.0 20 0.0 0.0 0.0 0.06515506906437321 0.0 21 0.0 0.0 0.0 0.06841282251759187 0.0 22 0.0 0.0 0.0 0.08470158978368517 0.0 23 0.0 0.0 0.0 0.0863304665102945 0.0 24 0.0 0.0 0.0 0.09773260359655982 0.0 25 0.0 0.0 0.0 0.10099035704977848 0.0 26 0.0 0.0 0.0 0.10424811050299713 0.0 27 0.0 0.0 0.0 0.13845452176179307 0.0 28 0.0 0.0 0.0 0.15962991920771435 0.0 29 0.0 0.0 0.0 0.20360959082616628 0.0 30 0.0 0.0 0.0 0.23130049517852488 0.0 31 0.0 0.0 0.0 0.2573625228042742 0.0 32 0.0 0.0 0.0 0.3176309616888194 0.0 33 0.0 0.0 0.0 0.3827860307531926 0.0 34 0.0 0.0 0.0 0.47726088089653373 0.0 35 0.0 0.0 0.0 0.5815089913995309 0.0 36 0.0 0.0 0.0 0.7786030753192599 0.0 37 0.0 0.0 0.0 0.99687255668491 0.0 38 0.0 0.0 0.0 1.1907088871514204 0.0 39 0.0 0.0 0.0 1.4480714099556946 0.0 40 0.0 0.0 0.0 1.6174745895230649 0.0 41 0.0 0.0 0.0 1.7868777690904352 0.0 42 0.0 0.0 0.0 1.9856007297367735 0.0 43 0.0 0.0 0.0 2.150117279124316 0.0 44 0.0 0.0 0.0 2.4042220484753716 0.0 45 0.0 0.0 0.0 2.604573885848319 0.0 46 0.0 0.0 0.0 2.767461558509252 0.0 47 0.0 0.0 0.0 2.935235861350013 0.0 48 0.0 0.0 0.0 3.078577013291634 0.0 49 0.0 0.0 0.0 3.2040005212405527 0.0 50 0.0 0.0 0.0 3.348970549908783 0.0 51 0.0 0.0 0.0 3.506971592389888 0.0 52 0.0 0.0 0.0 3.6568282512379464 0.0 53 0.0 0.0 0.0 3.7952827729997396 0.0 54 0.0 0.0 0.0 3.907675267135783 0.0 55 0.0 0.0 0.0 4.024954391451655 0.0 56 0.0 0.0 0.0 4.138975762314308 0.0 57 0.0 0.0 0.0 4.274172530622883 0.0 58 0.0 0.0 0.0 4.410998175658066 0.0 59 0.0 0.0 0.0 4.52339066979411 0.0 60 0.0 0.0 0.0 4.6455564242898095 0.0 61 0.0 0.0 0.0 4.774237685691947 0.0 62 0.0 0.0 0.0 4.8882590565546 0.0 63 0.0 0.0 0.0 5.0299713317696115 0.0 64 0.0 0.0 0.0 5.122817305186343 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCTGA 15 0.0022148523 70.0 15 TAAACAA 25 2.8090453E-6 70.0 5 GGATCAT 15 0.0022148523 70.0 57 GATCATT 15 0.0022148523 70.0 58 TAATAAA 15 0.0022148523 70.0 2 TTGTGCG 50 0.0 70.0 2 GGGTGTA 15 0.0022148523 70.0 34 CGCGTGT 15 0.0022148523 70.0 7 TGTGCGC 50 0.0 70.0 3 CTTGTGC 50 1.2732926E-11 62.999996 1 TGGCGCT 85 0.0 61.764706 13 TGCGCGT 35 2.929446E-7 60.0 5 GTCTGGC 35 2.929446E-7 60.0 10 TTGGGCG 30 8.286319E-6 58.333332 2 TTGTGGG 30 8.286319E-6 58.333332 2 GCGTCGG 25 2.3731786E-4 55.999996 8 GGTGTAG 25 2.3731786E-4 55.999996 35 CGCTGGC 45 2.647539E-8 54.444443 16 CGTCTGG 45 2.647539E-8 54.444443 9 TGCCGGC 65 3.6379788E-12 53.846153 19 >>END_MODULE