SRR1781252_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781252_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61392
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	15470	25.198722960646336	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	3476	5.661975501694032	No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	2862	4.661845191555903	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1761	2.8684519155590307	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1750	2.8505342715663278	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	523	0.8519025280166798	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	481	0.7834897054990878	RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT	346	0.5635913474068283	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	333	0.542415949960907	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	315	0.513096168881939	No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	255	0.4153635652853792	No Hit
CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT	252	0.4104769351055512	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT	211	0.34369298931456865	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT	211	0.34369298931456865	No Hit
CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT	200	0.32577534532186603	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTAGATCTCGTATGCCGT	177	0.28831118060985145	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCG	176	0.2866823038832421	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	174	0.28342455043002346	No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	156	0.2541047693510555	No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT	154	0.25084701589783687	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	150	0.24433150899139952	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	143	0.23292937190513419	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGA	140	0.22804274172530622	No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	140	0.22804274172530622	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	140	0.22804274172530622	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	136	0.2215272348188689	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACG	123	0.20035183737294762	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	123	0.20035183737294762	No Hit
CTTATACAAATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	119	0.19383633046651028	No Hit
CTTATACACATCTCCGAGCACACGAGACAATGGTAGATCTCGTATGCCGT	117	0.19057857701329164	No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGGTAGATCTCGTATGCCGT	114	0.18569194683346366	No Hit
CTTATACACATCTCAGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	109	0.177547563200417	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	109	0.177547563200417	No Hit
ATTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT	105	0.17103205629397966	No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	105	0.17103205629397966	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAG	98	0.15962991920771435	No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	96	0.1563721657544957	No Hit
ATTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT	90	0.14659890539483972	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCC	89	0.1449700286682304	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGAATGCCGT	87	0.14171227521501173	No Hit
CTTATACACATCTCCGCGCCCACGAGACAATGGTAGATCTCGTATGCCGT	86	0.1400833984884024	No Hit
CTTATACACATATCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	86	0.1400833984884024	No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	85	0.13845452176179307	No Hit
TCTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCG	68	0.11076361740943445	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTAAGCCGT	68	0.11076361740943445	No Hit
TCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	67	0.10913474068282514	No Hit
ATTATACAAATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	62	0.10099035704977848	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCTA	15	0.0022085088	70.050575	47
TGCCTTC	15	0.0022085088	70.050575	45
ATGCCTT	15	0.0022085088	70.050575	44
CGTAATC	15	0.0022085088	70.050575	48
GCCGTAT	20	7.840894E-5	70.050575	46
GAAAGCC	15	0.0022085088	70.050575	42
CGACACA	15	0.0022156702	69.993484	23
TAGAAAT	30	1.0088479E-7	69.993484	34
ACACGTA	20	7.872671E-5	69.993484	38
GTAGAAA	30	1.0088479E-7	69.993484	33
CAATGAT	20	7.872671E-5	69.993484	28
TCAAGTA	15	0.0022156702	69.993484	38
ATCTCCT	15	0.0022156702	69.993484	10
TATACGA	15	0.0022156702	69.993484	11
CTTTACA	20	7.872671E-5	69.993484	1
TATATCT	20	7.872671E-5	69.993484	34
GCAAGAT	15	0.0022156702	69.993484	32
GCAAGAA	30	1.0088479E-7	69.993484	56
AAATCGA	15	0.0022156702	69.993484	37
GAAATCG	20	7.872671E-5	69.993484	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content