FastQCFastQC Report
Wed 25 May 2016
SRR1781251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133743
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7621456.985412320644826No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG27072.0240311642478486No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9580.716299170797724No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7110.531616607972006No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260.31852134317310066No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4020.3005764787689823No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.20262742722983634No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700.20187972454633138No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT2580.19290729234427223No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2360.17645783330716375No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2010.15028823938449115No Hit
CTCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGG1790.13383878034738267No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1550.11589391594326431No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480.1106599971587298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT700.070.000015
CGACAAG150.002220595370.064
AACGTTG150.002220595370.045
TGCACAT150.002220595370.05
ACGTTGT150.002220595370.046
TATGCAC150.002220595370.03
GCGCGTC550.070.06
CGTAGCA150.002220595370.056
TTATACG150.002220595370.02
AGCGGTA150.002220595370.059
TATCATA150.002220595370.058
CTTGTAC150.002220595370.01
ATGCACA150.002220595370.04
AAAATAG150.002220595370.070
TGGAACA150.002220595370.032
CGTTGTA150.002220595370.047
ATCGAGT150.002220595370.020
GTTATAC150.002220595370.01
GAAAGGC150.002220595370.026
TGTCGGG207.9030586E-570.043
Produced by FastQC (version 0.11.5)