FastQCFastQC Report
Wed 25 May 2016
SRR1781251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133743
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3923529.33611478731597No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG82326.155088490612593No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT66624.981195277509851No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49383.6921558511473496No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC45273.3848500482268227No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC11230.8396701135760376No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT10130.7574228183904952Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT8260.6176024165750731No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT6520.48750214964521504No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC6420.48002512281016574No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5610.4194612054462664No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT5420.4052548544596727No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT5220.390300800789574No Hit
CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT4500.33646620757721896No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT4280.32001674854011053No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT3810.28487472241537876No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT3720.2781453982638344No Hit
CTTATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT3720.2781453982638344No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCG3420.25571431775868647No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3390.25347120970817166No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3140.23477864262054837No Hit
CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT3020.2258062104184892No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGA2950.22057229163395467No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAAACAGATCTCGTATGCCGT2870.2145906701659152No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2860.2138429674824103No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT2750.20561823796385603No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2540.1899164816102525No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACG2530.18916877892674758No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAG2520.18842107624324264No Hit
CTTATACACATCTCAGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2490.18617796819272783No Hit
CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT2440.18243945477520318No Hit
CTTATACAAATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2360.17645783330716375No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2330.17421472525664894No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2250.16823310378860948No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2110.15776526621954046No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT2090.1562698608525306Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTAAGCCGT1870.1398204018154221No Hit
ATTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT1850.13832499644841226No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGAATGCCGT1850.13832499644841226No Hit
CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1850.13832499644841226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCC1830.13682959108140239No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1790.13383878034738267No Hit
ATTATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT1760.13159567229686786No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1680.12561405082882843No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1600.11963242936078898No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCACGTATGCCGT1590.11888472667728404No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATG1490.11140769984223473No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT1430.10692148374120515No Hit
TATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1410.10542607837419529No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACT150.002201486470.1526149
GTATTAA150.002201486470.1526149
CCTTCTT252.7909045E-670.1526147
TATTAAG150.002201486470.1526150
AGTCTTA252.7909045E-670.1526148
TCTTCAT150.002201486470.1526150
ACGTCTA207.8182056E-570.152647
TGCCTTC207.8182056E-570.152645
AAGTCTA207.8182056E-570.152647
CTTCATA150.002204763570.1263251
CTTCAGA150.002204763570.1263251
ACTGATT150.002204763570.1263253
TCTGCAA301.004737E-770.1263253
AATACTA150.002204763570.1263253
TTCAGAT150.002204763570.1263252
ACTGCTA455.456968E-1270.1263153
TTCTGAA252.797131E-670.1263152
ATGCCTT207.847307E-570.1000444
ATACCGT150.002208044570.1000444
GTATCCC150.00221132970.07378442
Produced by FastQC (version 0.11.5)