FastQCFastQC Report
Wed 25 May 2016
SRR1781250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335329
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6452819.243191015390853No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG22490.6706846112325507No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7710.22992344831493844No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5510.16431623867902864No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4020.11988226488016246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA3750.049.46666756
GCCGTAT3650.048.90411454
GCTCTAA451.682196E-646.6666681
GTATCAT4000.046.37499657
CCGTATC3950.046.0759555
GTAGATC4600.042.608738
CGCCGTA4150.042.16867453
ATCATTA4400.041.36363659
AGATCTC4500.041.2222240
TAGATCT4500.041.2222239
TGGTCGC4550.040.76923449
ATCTCGG4400.040.5681842
TCTCGGT4400.040.5681843
GATCTCG4500.040.44444741
TATCATT4300.039.06976758
TCGGTGG4700.038.72340445
CGGTGGT4900.037.85714346
TCATTAA4800.037.18750460
CATTAAA4850.036.80412361
GTGGTCG5350.036.63551348
Produced by FastQC (version 0.11.5)