FastQCFastQC Report
Wed 25 May 2016
SRR1781250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335329
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT325109.694956296651945No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG67192.0037038251985364No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT56281.6783517083222745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC40531.2086637302470111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37741.1254618598451072No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT9840.29344315582606934RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC9010.2686913449179761No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT5840.17415732012441512No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT5710.17028053046411137No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5640.16819302833933242RNA PCR Primer, Index 32 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4980.1485108654485595No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT4980.1485108654485595No Hit
CTTATACACATCTCCGAGCCAACGAGACATCACTCGATCTCGTATGCCGT4060.12107512323717902No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT3950.11779476275538352No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT3770.11242690014880909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36400.061.62043470
ATTATAC12400.059.824941
TTCGTTA200.00693360552.52708454
CGTTACA200.00694589152.5035856
TCGATAT3200.052.4879234
GTCGTTT200.006954090752.4879233
TATGACG2900.051.93107243
ACGTCTT2500.050.4485947
CGTATGA3200.050.34596641
TATCGTA3150.049.98849538
ATATCGT3100.049.6659937
TCTTTAT2400.049.5719261
CGATATC3200.049.20742435
TCGAATG1000.049.0033440
TTTATAC2800.048.7387853
CTCGATA3600.048.59992633
ATCGTAT3250.048.45038639
ACGATCT800.048.11392634
TCGTATG76200.047.9100940
GATATCG3300.047.7162936
Produced by FastQC (version 0.11.5)