FastQCFastQC Report
Wed 25 May 2016
SRR1781249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435168
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9677922.23945694536363No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG38480.8842561953084785No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10830.2488694021619237No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10800.2481800132362674No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5520.1268475623207589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTTT200.006949465352.49939311
CTGTTTA200.006949465352.49939350
TACCGTA200.006949465352.49939337
GCCGTAT6550.051.2971254
CCGTATC6500.050.61480355
TATCATT6550.049.699858
ATCATTA6700.049.63199659
CGTATCA6750.049.25869456
TCATTAA6950.048.35032360
TAGATCT6950.048.3447739
CATTAAA7100.047.3288461
GTATCAT7250.046.83242857
CTGTTTT305.872359E-446.67149461
TATAGAC305.875687E-446.666133
TTGTGCG1450.045.8615462
GTAGATC7500.045.73280738
TGTAGAT8000.044.62448537
TCTCGGT7850.044.58547243
AGATCTC7750.044.25755740
ATTAAAA7650.043.92611362
Produced by FastQC (version 0.11.5)