FastQCFastQC Report
Wed 25 May 2016
SRR1781249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435168
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5041811.585870284579748No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG105502.4243510552246486No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT79271.8215953378924918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68091.5646830649312449No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC55821.282722994337819No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC13710.3150507390249283No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT13670.31413155379071994RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT10930.25116736524744465No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT9160.21049341863372306No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT8500.1953268622692845No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT8050.18498602838444003No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC7670.17625376865946027No Hit
CTTATACACATCTCCGAGCCAACGAGACATAAGTTAATCTCGTATGCCGT7210.16568313846606367No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6720.1544231193470108No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT6340.14569085962203104No Hit
CTTATACACATCTCCGAGACCACGAGACATAAGTTAATCTCGTATGCCGT5340.12271122876682108No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT4940.11351937642473711No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG4380.10065078314581954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG150.002226160669.9826533
TCTTTAT2150.063.4799271
ATTATAC17250.063.093061
AGGGGGG58100.061.43055770
TTATGCC308.321065E-658.39267742
GATAATC3650.056.56131733
CCGAAAT252.374702E-456.0698747
GACAGTA252.3923094E-455.98611526
TTAATCG252.3923094E-455.98611534
AGATAAT3950.055.80894532
ATAATCT3650.053.68531834
CCGTATA200.006914959352.5655147
ATCTAGC200.006955963652.486998
ATATCGA407.4863055E-752.4869937
TAACTCG200.006955963652.4869936
AGGCTAA200.006955963652.4869970
TAAGCCG1550.051.9883243
TATCGTA5000.051.7871638
AATATCG5350.051.6694336
ATCGTAT5450.050.72719639
Produced by FastQC (version 0.11.5)