FastQCFastQC Report
Wed 25 May 2016
SRR1781248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134101
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7385555.074160520801485No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG21621.6122176568407396No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9700.7233353964549109No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6110.45562672910716545No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4230.31543388938188377No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.24384605633067613No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480.18493523538228648No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.18269811559943624No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT2290.1707668100909016No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2000.14914131885668264No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1640.12229588146247976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTA150.002220609270.0000144
AATCTGG150.002220609270.0000127
CGCCTCT150.002220609270.0000145
AACTTAT150.002220609270.0000145
TTGTGGG301.01739715E-770.000012
GTTGTGG301.01739715E-770.000011
CACGGCT150.002220609270.000017
ACGTCTG207.903145E-570.08
TGAACAT207.903145E-570.029
TTGTGCG353.6798156E-970.02
CGTCTGG950.062.6315849
TTGGGCG401.0575604E-861.252
CTTGTGC352.9560397E-760.01
TGTGGGC352.9560397E-760.03
GTCTGGC1050.059.99999610
CGCGTCT750.056.07
ACATGTG252.3834022E-455.99999632
TACACGT252.3834022E-455.9999965
TGTGCGC509.713403E-1055.9999963
AATGAAC252.3834022E-455.99999627
Produced by FastQC (version 0.11.5)