FastQCFastQC Report
Wed 25 May 2016
SRR1781248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134101
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3844828.670927137008672No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG82486.150587989649592No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT63144.7083914363054715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40142.993266269453621No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC39722.9619465924937174No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC10940.8158030141460542No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT9600.7158783305120767Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT8340.6219192996323667No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC6940.5175203764326888No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT6050.451152489541465No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT5590.416849986204428No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5420.40417297410161No Hit
CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT4630.3452621531532203No Hit
CTTATACACATCTCCGAGACCACGAGACTTGCTAAGATCTCGTATGCCGT3820.28485991901626384No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG3450.25726877502777756No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT3410.2542859486506439No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3270.24384605633067613No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC3260.24310034973639272No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT3060.22818621785072446No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCG3000.22371197828502395No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2960.22072915190789033No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT2920.21774632553075668No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT2890.21550920574790644No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGA2790.2080521398050723No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2610.19462942110797085No Hit
CTTATACAAATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2540.18940947494798696No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT2530.18866376835370355No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2470.18418952878800307No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAG2470.18418952878800307No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2410.1797152892223026No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCAAAGATCTCGTATGCCGT2320.17300392987375188No Hit
CTTATACACATCTCAGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2290.1707668100909016No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACG2250.167783983713768No Hit
CTTATACACATCTCCGAGCACACGAGACTTGCTAAGATCTCGTATGCCGT2230.16629257052520116No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTTAGATCTCGTATGCCGT2180.1625640375537841No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1960.14615849247954898No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1950.14541278588526557No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTAAGCCGT1880.1401928397252817No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTAAGATCTCGTATGCCGT1850.13795571994243144No Hit
ATTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT1790.13348148037673097No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1770.13199006718816414No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCC1770.13199006718816414No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1690.12602441443389684No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1590.1185673484910627No Hit
ATTATACACATCTCCGAGACCACGAGACTTGCTAAGATCTCGTATGCCGT1520.11334740233107882No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCACGTATGCCGT1510.1126016957367954No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGAATGCCGT1500.11185598914251198No Hit
CTTATACACATATCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1490.11111028254822856No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1410.10514462979396126No Hit
TCTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCG1370.10216180341682762No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAA207.802419E-570.1813847
ACGTATT252.784156E-670.1813747
GACGTAT252.784156E-670.1813746
GACGAAT150.002197922270.1813746
AATCCCG150.002201185670.1551443
TAAGCAG150.002201185670.1551443
GAATCCC150.002201185670.1551442
TATGCTA150.002214275570.050453
TCTGAAT301.0123222E-770.050453
AGTTCGA150.002214275570.050453
CTTCAAA252.8215E-670.02425457
TTGTAAA207.8895755E-570.02425458
TGATAGA252.8215E-670.02425455
CTGAATG301.0149597E-770.02425454
ATCAGAT207.8895755E-570.02425454
TGCTTCA207.8895755E-570.02425455
TGCTTAA252.8215E-670.02425455
CGAATCC150.00221755770.02425441
ATGCTAG150.00221755770.02425454
CGTATAC150.00221755770.02425441
Produced by FastQC (version 0.11.5)