FastQCFastQC Report
Wed 25 May 2016
SRR1781247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153743
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7377447.98527412630168No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG20831.3548584325790443No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9550.6211664921329751No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5750.3740007675146185No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.25236921355769043No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.21789609933460385No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2290.14894987088843067No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100.13659158465751287No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT1930.1255341706614285No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1810.11772893725242775No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG1570.10211847043442629No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGAT150.0022186570.020528
GTAGATA207.908094E-569.9977238
TGTGACG150.00222151669.9977211
GTATCAA150.00222151669.9977257
TCAATAA252.82984E-669.9977260
AGCAGTT150.00222151669.9977246
CCGTAAC150.00222151669.9977256
CGTAACA150.00222151669.9977257
AAAAATG207.908094E-569.9977270
TAGATAT207.908094E-569.9977239
ATGAGAA207.908094E-569.9977262
TTGTGCG550.063.6342932
TTGTGGG401.0591975E-861.248012
CAATAAA308.347357E-658.33143661
ATCAATA308.347357E-658.33143659
AATAAAA308.347357E-658.33143662
GGCAACC252.381054E-456.016430
TATCGGT252.3848869E-455.99817743
ACATTAA252.3848869E-455.99817761
CTTATGC252.3848869E-455.9981771
Produced by FastQC (version 0.11.5)