FastQCFastQC Report
Wed 25 May 2016
SRR1781247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153743
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2830318.40929343124565No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG60513.9357889464886204No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT44692.9067990087353572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39572.5737757166179924No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC30922.0111484750525226No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT16571.077772646559518No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT16281.058909999154433No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT8390.5457159025126347No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC7460.48522534359287905No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT6750.43904437925629136No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA5690.37009815081011815No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT5050.3284702392954476No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT4660.3031032307161952No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4460.29009450836786066RNA PCR Primer, Index 30 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC4320.2809884027240265No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT4290.27903709437177626No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4170.2712318609627755No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT4060.2640770636711915No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCG3950.2569222663796075No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACG3860.251068341322857No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCGT3530.22960394944810497No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCCT3200.20813955757335292No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGAATGCCGT3150.20488737698626927No Hit
CTTATACACATCTCCGAGCCAACGAGACATTGCCGGATCTCGTATGCCGT2900.1886264740508511No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGACGT2750.1788699322896002No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT2640.17171513499801616No Hit
CTTATACACATCTCCGAGACCACGAGACATTGCCGGATCTCGTATGCCGT2480.1613081571193485No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2450.15935684876709832No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT2150.13984376524459652No Hit
CTTATACACATCTCCGCGCCCACGAGACATTGCCGGATCTCGTATGCCGT2020.13138809571817903No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1930.1255341706614285No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1910.12423329842659503No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCG1800.11707850113501102No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1790.1164280650175943No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCGCGTATGCCGT1790.1164280650175943No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT1730.11252544831309394No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA1650.1073219593737601No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1600.10406977878667646No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCC1570.10211847043442629No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCTC150.002209737470.0915645
AAGTCTT252.8073919E-670.0915647
ACTATGA207.868449E-570.0687341
CCTAAGC207.868449E-570.0687341
CCAAAGC150.002212595770.0687341
TCTGATA207.881159E-570.0459153
TGATAGA150.002215456770.0459155
AGCTTTA150.002215456770.0459155
ATATGAT252.818284E-670.0459152
CTAAATG150.002215456770.0459154
TTAAGAA301.01377736E-770.0459152
ATAAGAA252.818284E-670.0459152
AATTATG207.881159E-570.0459150
TAAGCTA207.881159E-570.0459153
CTGATAG207.881159E-570.0459154
CATCAGA207.881159E-570.0459151
TAAGAAT500.070.0459153
AAAGCTA150.002215456770.0459153
ATACATG150.002215456770.0459154
GACTACT207.881159E-570.0459149
Produced by FastQC (version 0.11.5)