##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781247_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 153743 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.128669272747377 32.0 27.0 32.0 13.0 32.0 2 30.088908112889694 32.0 32.0 32.0 27.0 32.0 3 29.991947600866382 32.0 32.0 32.0 27.0 32.0 4 29.456918363762902 32.0 32.0 32.0 22.0 32.0 5 30.133599578517394 32.0 32.0 32.0 27.0 32.0 6 33.60672030596515 37.0 32.0 37.0 22.0 37.0 7 33.582803769927736 37.0 32.0 37.0 22.0 37.0 8 31.907260818378724 37.0 32.0 37.0 13.0 37.0 9 32.467800160007286 37.0 32.0 37.0 13.0 37.0 10-11 33.19808706737868 37.0 34.5 37.0 20.0 37.0 12-13 33.54819406412 37.0 32.0 37.0 24.5 37.0 14-15 33.2118535478038 37.0 32.0 37.0 22.0 37.0 16-17 32.010608613075064 37.0 29.5 37.0 13.0 37.0 18-19 32.54437275193017 37.0 32.0 37.0 17.5 37.0 20-21 32.802254411582965 37.0 32.0 37.0 17.5 37.0 22-23 32.60698048041212 37.0 32.0 37.0 17.5 37.0 24-25 33.38849898857184 37.0 32.0 37.0 24.5 37.0 26-27 33.779534678001596 37.0 37.0 37.0 24.5 37.0 28-29 33.51579584111147 37.0 34.5 37.0 22.0 37.0 30-31 33.22180847258087 37.0 34.5 37.0 22.0 37.0 32-33 32.610538365974385 37.0 32.0 37.0 13.0 37.0 34-35 31.928068269774883 37.0 32.0 37.0 13.0 37.0 36-37 32.01856669897166 37.0 32.0 37.0 13.0 37.0 38-39 30.434966144800086 37.0 27.0 37.0 13.0 37.0 40-41 26.551547062305275 32.0 13.0 37.0 8.0 37.0 42-43 27.296124051176314 34.5 13.0 37.0 8.0 37.0 44-45 29.395260272012386 37.0 22.0 37.0 10.5 37.0 46-47 26.582270412311455 32.0 13.0 37.0 8.0 37.0 48-49 26.681507450745727 34.5 17.5 37.0 8.0 37.0 50-51 26.10333153379341 29.5 13.0 37.0 8.0 37.0 52-53 26.969218110743256 32.0 17.5 37.0 10.5 37.0 54-55 26.23289190402165 32.0 13.0 37.0 8.0 37.0 56-57 25.441337166570186 29.5 13.0 37.0 8.0 37.0 58-59 26.838672329797127 32.0 17.5 37.0 10.5 37.0 60-61 27.177136520036683 34.5 17.5 37.0 8.0 37.0 62-63 31.22883968701014 37.0 29.5 37.0 13.0 37.0 64-65 32.424780315201346 37.0 32.0 37.0 13.0 37.0 66-67 32.43498891006419 37.0 32.0 37.0 13.0 37.0 68-69 31.66001378924569 37.0 29.5 37.0 13.0 37.0 70-71 27.97319227542067 32.0 20.0 37.0 10.5 37.0 72-73 27.95116525630435 34.5 17.5 37.0 10.5 37.0 74-75 31.296426503970913 37.0 29.5 37.0 13.0 37.0 76 32.485869275349124 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.8845650092535493 11101 2 -0.2996193887260077 11101 3 -0.5905440573494758 11101 4 -0.5690347286009896 11101 5 -0.22869629467812302 11101 6 1.157566180295099 11101 7 0.501770290280291 11101 8 -0.23596385752573568 11101 9 1.1385779064796608 11101 10-11 -0.7340971962932699 11101 12-13 -0.18254009071038269 11101 14-15 1.0954738529320522 11101 16-17 1.1885534155930166 11101 18-19 0.05353039785788383 11101 20-21 0.7656803756548101 11101 22-23 0.634333472520261 11101 24-25 -0.1843389174057961 11101 26-27 -0.14198438034004823 11101 28-29 0.10739209705779018 11101 30-31 0.5081484864915282 11101 32-33 -0.6141236718616838 11101 34-35 -0.7348031125667944 11101 36-37 -0.265851673135721 11101 38-39 0.4457237148059505 11101 40-41 0.08447245446000196 11101 42-43 -0.23069938055363437 11101 44-45 0.0033323074256941254 11101 46-47 1.186264792958422 11101 48-49 -0.5122112503223448 11101 50-51 0.39620396362202115 11101 52-53 -0.4733653854756277 11101 54-55 -1.2664213840416174 11101 56-57 -1.406190667901079 11101 58-59 -1.3219433603621127 11101 60-61 0.5908611292146553 11101 62-63 -1.0189948470068018 11101 64-65 -0.17831165069925703 11101 66-67 -0.42255781566021255 11101 68-69 -0.6394264283844571 11101 70-71 -1.5141376418885457 11101 72-73 -0.9859586216484431 11101 74-75 0.17202422510384707 11101 76 0.2190010352382643 11102 1 0.7863179272543874 11102 2 -0.6227840712656914 11102 3 -0.3777464383018554 11102 4 -0.9571795698708314 11102 5 -1.3990831994400281 11102 6 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0.06308050579646363 21611 38-39 1.0159257350079685 21611 40-41 2.2020049219924687 21611 42-43 1.009271759417505 21611 44-45 1.320215424308806 21611 46-47 0.8576212143148432 21611 48-49 -0.19388513199622537 21611 50-51 1.8428129102309683 21611 52-53 1.4017428396325968 21611 54-55 2.125642108021875 21611 56-57 1.546118134407724 21611 58-59 0.8464549080361579 21611 60-61 0.32700831536184083 21611 62-63 0.61303978502783 21611 64-65 0.4890043666167543 21611 66-67 1.455725012622615 21611 68-69 1.0638924749344447 21611 70-71 1.4106098328589276 21611 72-73 2.472771537081716 21611 74-75 0.6547803578599805 21611 76 -0.18402926779203455 21612 1 1.4203639798859662 21612 2 1.0792444375062402 21612 3 0.6688544388911275 21612 4 0.4142568336379391 21612 5 0.11215583564769105 21612 6 -0.14276798846012184 21612 7 0.3543183270388539 21612 8 0.8129083229253915 21612 9 1.8286363860118229 21612 10-11 1.208676404375069 21612 12-13 0.24644904880507568 21612 14-15 0.2164011712277869 21612 16-17 1.0218867489263452 21612 18-19 0.4138060870809426 21612 20-21 1.345254310491896 21612 22-23 0.9829132553105708 21612 24-25 1.2454856439977107 21612 26-27 1.0328276497351396 21612 28-29 0.22978140365763267 21612 30-31 0.3525511598415605 21612 32-33 0.413236394972067 21612 34-35 0.7315711564390526 21612 36-37 0.7013580177581815 21612 38-39 0.9296418434609173 21612 40-41 1.4579060384199032 21612 42-43 0.4670867514430235 21612 44-45 0.8466907034156677 21612 46-47 2.293825361045304 21612 48-49 0.43306861601015356 21612 50-51 1.6777411398960425 21612 52-53 2.0184892511158523 21612 54-55 2.094063160653455 21612 56-57 2.218287193418888 21612 58-59 2.1968536321190903 21612 60-61 0.30890624199660977 21612 62-63 0.4120828472287883 21612 64-65 0.8561495021829444 21612 66-67 1.6373837048076254 21612 68-69 1.194115760420889 21612 70-71 1.57065767974888 21612 72-73 0.9973329405904892 21612 74-75 1.2373959878440317 21612 76 0.5523343685716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 4.0 17 16.0 18 47.0 19 173.0 20 461.0 21 946.0 22 1785.0 23 2914.0 24 4273.0 25 5956.0 26 8003.0 27 10250.0 28 12421.0 29 14797.0 30 16530.0 31 17928.0 32 18030.0 33 17524.0 34 14418.0 35 6600.0 36 666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.732153426867264 19.24730553333203 13.816093299770394 48.20444774003031 2 11.406698191137158 8.753569268194324 58.70966483026869 21.13006771039982 3 12.210637232264233 9.590680551309653 60.3624230046246 17.836259211801515 4 10.575440833078579 49.25492542749914 23.564650097890635 16.60498364153165 5 11.52963061732892 21.2419427225955 57.24228094937656 9.986145710699024 6 18.139362442517708 58.418269449666006 13.069212907254313 10.373155200561976 7 16.56660791060406 19.570972336951925 10.109728573008201 53.75269117943581 8 14.415615670306941 60.01378924568923 5.8012397312397965 19.76935535276403 9 12.735539179019534 16.72791606772341 4.5413449718035945 65.99519978145346 10-11 15.67420955750831 36.19514384394737 28.580162999291026 19.550483599253297 12-13 14.926630328732552 9.16015142251304 35.71730561590197 40.19591263285244 14-15 15.436149938533786 6.940153372836487 12.347879253039162 65.27581743559057 16-17 42.73788074904223 28.69561541013249 4.747533221024697 23.818970619800574 18-19 45.070669884157326 14.637089168287337 4.615169471130393 35.67707147642494 20-21 18.19692603890909 8.403309418965417 4.6324060282419355 68.76735851388356 22-23 16.24041419771957 34.17781622577938 4.516628399341759 45.06514117715928 24-25 42.98309516530834 32.17772516472295 4.479228322590297 20.359951347378416 26-27 44.88627124486968 37.1415283947887 5.343657922637128 12.628542437704482 28-29 18.466857027637033 36.56101415999428 6.32321471546672 38.64891409690197 30-31 15.486558737633583 14.242924881132799 54.11108148013243 16.15943490110119 32-33 42.43217577385637 6.6767267452827115 15.802345472639404 35.08875200822151 34-35 41.276294051072576 7.50478073655865 5.962911891350444 45.25601332101833 36-37 49.33395341576527 28.081603715291102 6.68323110645688 15.901211762486748 38-39 17.74682424565671 14.774981625179684 32.901660563407766 34.57653356575584 40-41 15.406330085467863 9.79335510140365 35.50786382380872 39.292450989319775 42-43 41.07610951027391 8.221401205939937 31.349885195038347 19.352604088747814 44-45 17.002357915277667 34.58427514432068 35.51540775672819 12.897959183673468 46-47 39.35595326789624 12.452784763590342 15.794370935260224 32.39689103325319 48-49 37.784503237983664 8.457808584724527 7.849588336750301 45.9080998405415 50-51 19.987505775325207 12.601922288525486 32.73389253665298 34.67667939949632 52-53 12.925006263849614 16.27689794643351 51.67789821000328 19.120197579713587 54-55 13.109079670776538 20.19193857965451 33.88203910341911 32.81694264614984 56-57 38.25287685312274 11.388045040884165 14.625927781066435 35.733150324926655 58-59 16.38937949473561 17.168710948045653 47.26306990238841 19.178839654830327 60-61 38.37797754563076 32.688604993039924 16.403007792680864 12.530409668648446 62-63 15.287900222775095 66.23022261248515 6.094606240954843 12.3872709237849 64-65 12.291616528882615 69.72382482454485 5.5631801122652735 12.421378534307253 66-67 12.558295337023473 69.75894837488536 5.247393377259453 12.435362910831712 68-69 13.567121755136819 69.04184255543342 5.378131036860215 12.012904652569548 70-71 23.922715180528545 58.25923781895761 5.725463923560747 12.092583076953096 72-73 67.91821416259603 13.852012774565345 6.125156917713326 12.104616145125306 74-75 75.85255914090398 6.161906558347372 5.927099119959934 12.05843518078872 76 76.6116180899293 5.434393761016762 5.744001352907125 12.209986796146817 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 1.5 15 0.5 16 1.5 17 3.0 18 5.5 19 8.0 20 11.0 21 14.0 22 14.0 23 19.0 24 36.0 25 48.0 26 47.0 27 62.0 28 93.5 29 109.0 30 124.0 31 170.5 32 242.0 33 282.0 34 328.0 35 506.5 36 736.0 37 833.0 38 1026.5 39 1449.5 40 2066.5 41 3066.0 42 3678.0 43 4450.0 44 6540.0 45 9916.0 46 11974.0 47 15317.0 48 22533.5 49 21271.0 50 16135.0 51 9099.5 52 1600.0 53 912.0 54 688.0 55 712.5 56 911.5 57 1245.5 58 1405.0 59 1579.0 60 1926.0 61 2241.0 62 2383.0 63 2563.5 64 2791.5 65 2855.0 66 2873.0 67 2875.0 68 2888.0 69 2916.0 70 2914.5 71 2898.0 72 2877.5 73 2782.5 74 2620.5 75 2533.0 76 2449.5 77 2181.0 78 1760.5 79 1525.0 80 1382.0 81 1093.0 82 814.0 83 681.0 84 552.5 85 334.0 86 188.0 87 132.0 88 109.0 89 76.0 90 50.5 91 33.5 92 32.0 93 35.5 94 40.0 95 51.5 96 62.0 97 112.0 98 323.5 99 2021.5 100 3558.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013008722348334559 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.504361174167279E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 6.504361174167279E-4 36-37 0.0 38-39 0.0 40-41 6.504361174167279E-4 42-43 0.0026017444696669117 44-45 0.0035773986457920035 46-47 0.03805051286887858 48-49 0.06374273950683934 50-51 0.04683140045400441 52-53 0.054311415804296784 54-55 0.031220933636002937 56-57 0.010406977878667647 58-59 0.01398437652445965 60-61 0.005853925056750552 62-63 3.2521805870836396E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 153743.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.10803093474174 #Duplication Level Percentage of deduplicated Percentage of total 1 79.56565178524478 29.52524667789753 2 9.219820862035723 6.842587955223978 3 4.73260766682442 5.268532551075497 4 2.669541287619849 3.9624568273027063 5 1.4267935706648436 2.6472749978860826 6 0.772992585581322 1.721053966684662 7 0.3733501603828154 0.9698002510683413 8 0.2348775656868416 0.6972675178707324 9 0.13146132407845612 0.43904437925629136 >10 0.6853517028623513 4.925752717196881 >50 0.07887679444707367 2.082696447968363 >100 0.08764088271897075 6.776243471247471 >500 0.008764088271897074 2.168554015467371 >1k 0.008764088271897074 9.628405846119824 >5k 0.001752817654379415 3.9357889464886204 >10k+ 0.001752817654379415 18.40929343124565 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 28303 18.40929343124565 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 6051 3.9357889464886204 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 4469 2.9067990087353572 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 3957 2.5737757166179924 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 3092 2.0111484750525226 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT 1657 1.077772646559518 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT 1628 1.058909999154433 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT 839 0.5457159025126347 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 746 0.48522534359287905 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT 675 0.43904437925629136 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA 569 0.37009815081011815 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT 505 0.3284702392954476 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT 466 0.3031032307161952 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 446 0.29009450836786066 RNA PCR Primer, Index 30 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 432 0.2809884027240265 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT 429 0.27903709437177626 No Hit ATCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 417 0.2712318609627755 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT 406 0.2640770636711915 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCG 395 0.2569222663796075 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACG 386 0.251068341322857 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCGT 353 0.22960394944810497 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCCT 320 0.20813955757335292 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGAATGCCGT 315 0.20488737698626927 No Hit CTTATACACATCTCCGAGCCAACGAGACATTGCCGGATCTCGTATGCCGT 290 0.1886264740508511 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGACGT 275 0.1788699322896002 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT 264 0.17171513499801616 No Hit CTTATACACATCTCCGAGACCACGAGACATTGCCGGATCTCGTATGCCGT 248 0.1613081571193485 No Hit CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 245 0.15935684876709832 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT 215 0.13984376524459652 No Hit CTTATACACATCTCCGCGCCCACGAGACATTGCCGGATCTCGTATGCCGT 202 0.13138809571817903 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 193 0.1255341706614285 No Hit CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 191 0.12423329842659503 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCG 180 0.11707850113501102 No Hit TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 179 0.1164280650175943 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCGCGTATGCCGT 179 0.1164280650175943 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT 173 0.11252544831309394 No Hit ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA 165 0.1073219593737601 No Hit CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 160 0.10406977878667646 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCC 157 0.10211847043442629 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0013008722348334559 0.0 2 0.0 0.0 0.0 0.0019513083522501838 0.0 3 0.0 0.0 0.0 0.0019513083522501838 0.0 4 0.0 0.0 0.0 0.0019513083522501838 0.0 5 0.0 0.0 0.0 0.008455669526417462 0.0 6 0.0 0.0 0.0 0.00910610564383419 0.0 7 0.0 0.0 0.0 0.00910610564383419 0.0 8 0.0 0.0 0.0 0.00910610564383419 0.0 9 0.0 0.0 0.0 0.01235828623091783 0.0 10 0.0 0.0 0.0 0.01235828623091783 0.0 11 0.0 0.0 0.0 0.014309594583168014 0.0 12 0.0 0.0 0.0 0.0162609029354182 0.0 13 0.0 0.0 0.0 0.022765264109585476 0.0 14 0.0 0.0 0.0 0.023415700227002206 0.0 15 0.0 0.0 0.0 0.023415700227002206 0.0 16 0.0 0.0 0.0 0.023415700227002206 0.0 17 0.0 0.0 0.0 0.02536700857925239 0.0 18 0.0 0.0 0.0 0.028619189166336028 0.0 19 0.0 0.0 0.0 0.0442296559843375 0.0 20 0.0 0.0 0.0 0.052685325510754964 0.0 21 0.0 0.0 0.0 0.06439317562425606 0.0 22 0.0 0.0 0.0 0.0793532063248408 0.0 23 0.0 0.0 0.0 0.08260538691192444 0.0 24 0.0 0.0 0.0 0.08650800361642481 0.0 25 0.0 0.0 0.0 0.09171149255575864 0.0 26 0.0 0.0 0.0 0.10537065102150993 0.0 27 0.0 0.0 0.0 0.1509011792406809 0.0 28 0.0 0.0 0.0 0.19382996299018493 0.0 29 0.0 0.0 0.0 0.2666788081408584 0.0 30 0.0 0.0 0.0 0.31546151694711305 0.0 31 0.0 0.0 0.0 0.36554509798820106 0.0 32 0.0 0.0 0.0 0.4657122600703772 0.0 33 0.0 0.0 0.0 0.6198656198981417 0.0 34 0.0 0.0 0.0 0.7902798826613244 0.0 35 0.0 0.0 0.0 1.0146803431700955 0.0 36 0.0 0.0 0.0 1.2865626402502879 0.0 37 0.0 0.0 0.0 1.7041426276318272 0.0 38 0.0 0.0 0.0 2.1288774123049503 0.0 39 0.0 0.0 0.0 2.5145860299330702 0.0 40 0.0 0.0 0.0 2.93997125072361 0.0 41 0.0 0.0 0.0 3.3029146042421442 0.0 42 0.0 0.0 0.0 3.626181354598258 0.0 43 0.0 0.0 0.0 3.953350721658872 0.0 44 0.0 0.0 0.0 4.273365291427902 0.0 45 0.0 0.0 0.0 4.626552103185186 0.0 46 0.0 0.0 0.0 4.973884989885718 0.0 47 0.0 0.0 0.0 5.304956973650833 0.0 48 0.0 0.0 0.0 5.59570191813611 0.0 49 0.0 0.0 0.0 5.868885087451136 0.0 50 0.0 0.0 0.0 6.145320437353245 0.0 51 0.0 0.0 0.0 6.411999245494104 0.0 52 0.0 0.0 0.0 6.6981911371574645 0.0 53 0.0 0.0 0.0 6.946657734010654 0.0 54 0.0 0.0 0.0 7.178863427928426 0.0 55 0.0 0.0 0.0 7.423427408077115 0.0 56 0.0 0.0 0.0 7.627013912828551 0.0 57 0.0 0.0 0.0 7.846861320515406 0.0 58 0.0 0.0 0.0 8.07646526996351 0.0 59 0.0 0.0 0.0 8.302817038824532 0.0 60 0.0 0.0 0.0 8.489492204523133 0.0 61 0.0 0.0 0.0 8.690476964804901 0.0 62 0.0 0.0 0.0 8.892112161204087 0.0 63 0.0 0.0 0.0 9.048216829384103 0.0 64 0.0 0.0 0.0 9.212777167090534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCCTC 15 0.0022097374 70.09156 45 AAGTCTT 25 2.8073919E-6 70.09156 47 ACTATGA 20 7.868449E-5 70.06873 41 CCTAAGC 20 7.868449E-5 70.06873 41 CCAAAGC 15 0.0022125957 70.06873 41 TCTGATA 20 7.881159E-5 70.04591 53 TGATAGA 15 0.0022154567 70.04591 55 AGCTTTA 15 0.0022154567 70.04591 55 ATATGAT 25 2.818284E-6 70.04591 52 CTAAATG 15 0.0022154567 70.04591 54 TTAAGAA 30 1.01377736E-7 70.04591 52 ATAAGAA 25 2.818284E-6 70.04591 52 AATTATG 20 7.881159E-5 70.04591 50 TAAGCTA 20 7.881159E-5 70.04591 53 CTGATAG 20 7.881159E-5 70.04591 54 CATCAGA 20 7.881159E-5 70.04591 51 TAAGAAT 50 0.0 70.04591 53 AAAGCTA 15 0.0022154567 70.04591 53 ATACATG 15 0.0022154567 70.04591 54 GACTACT 20 7.881159E-5 70.04591 49 >>END_MODULE