FastQCFastQC Report
Wed 25 May 2016
SRR1781246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293633
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7695626.20822591466218No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG55401.8867089189566568No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8890.30275888609250323No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8550.291179806084466No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT4820.1641504871727633No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4290.14610074480729346No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.13077549185547946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC150.002223250570.055
AAGTGTA550.063.63636834
TTGTGCG900.062.2222182
CTTGTGC750.060.6666681
AGTGTAG700.060.035
CGTATAA308.364626E-658.33333256
CCGTATA252.3880957E-456.00000455
TAGATCT10900.052.6605539
CGTATCA10450.052.58373356
CGTATCG200.006946909252.556
GGTCCAA200.006946909252.511
AGGGTTC200.006946909252.55
TGTTCTA200.006946909252.548
TATAGAT200.006946909252.533
ACCGTTC200.006946909252.58
CTCTAGC200.006946909252.51
GCCGTAT10600.052.554
TGCAACG200.006946909252.512
CCGTATC10450.051.91387655
GTAGATC11300.051.41592838
Produced by FastQC (version 0.11.5)