FastQCFastQC Report
Wed 25 May 2016
SRR1781246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293633
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3903813.294827216286997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91843.127713846876884No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG88082.9996628444350604No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT57391.9544805931213451No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC46071.5689653410890465No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT15690.534340486253248No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC11560.3936887202732663No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT9260.31535964963066143Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT7220.24588516958243792No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT5840.198887727196875No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5730.19514155425309826Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTAAGCCGT5530.1883303307189587No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5370.18288135189164706No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGA4840.16483160952617723No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT4600.15665814128520977No Hit
CTTATACACATCTCCGAGCCAACGAGACAGTAGATTATCTCGTATGCCGT4320.1471224283374144No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACG3770.1283915636185306No Hit
CTTATACACATCTCCGAGACCACGAGACAGTAGATTATCTCGTATGCCGT3670.12498595185146083No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG3200.10897957654623289No Hit
CTTATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT3060.1042117200723352No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAG3020.10284947536550729No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2940.10012498595185146Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG207.927492E-569.98450536
ATTATAC12850.062.3597341
AGGGGGG41200.061.7460470
CTCGAAA352.9693365E-759.99693339
CCGAATA308.316583E-658.39002647
GTCATAT553.6379788E-1157.29908449
ACGTCTA252.3766575E-456.0544347
AATCCGA252.3907099E-455.987611
CGACACA252.3907099E-455.987623
TTATCGT252.3907099E-455.987635
ATATGCT700.055.01592652
TCTTTAT2550.054.88981
TATATCG1600.054.67539636
ATATCTC1350.054.4323935
GCCGTAT3100.054.2462246
GAATATC1750.053.9880433
CTTTATA2650.052.8184932
CCGTATT3000.052.55102547
GACGTAA200.00692026652.5510246
TTCCGTA200.00692026652.5510245
Produced by FastQC (version 0.11.5)