FastQCFastQC Report
Wed 25 May 2016
SRR1781245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370326
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7324719.779059531331853No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG26590.7180160183189946No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10290.277863288021905No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6370.1720106068707031No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4220.11395365164746736No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3900.10531261645145089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4450.055.84269754
CCGTATC4550.054.61538755
CGTATCA4750.053.78947456
TTACACG200.00694833652.551
TCATTAA4900.052.14285760
CGCCGTA5150.051.65048653
GTATCAT5150.050.29126457
TATCATT4950.050.2020258
ATCATTA4950.050.2020259
TTGTGCG651.8553692E-1048.4615362
TAGATCT5600.046.87539
GACGTAT305.8742135E-446.66666454
TACGCGT451.6826762E-646.6666645
CCATAAG305.8742135E-446.6666641
TGTAGGT451.6826762E-646.66666437
TGGTCGC6250.046.4849
TCGGTGG6450.046.12402745
CATTAAA5750.045.65217261
AGATCTC5650.045.2212440
ATCTCGG5850.044.87179642