FastQCFastQC Report
Wed 25 May 2016
SRR1781245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370326
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3764310.164827746364015No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG83172.245859053914659No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT58261.5732084703747509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48711.315327576243634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC47301.2772530149111865No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC11010.2973056172129421No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT10930.29514535841393796No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT9300.25113008538422904RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT6550.17687118916846237No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC6300.1701203804215745No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC5580.15067805123053743No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT4140.11179339284846325No Hit
CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT4030.10882303699983258No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGA3760.10153216355319368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG150.002224509869.9936538
ATTATAC13950.061.948971
AGGGGGG44150.061.73310570
TCGTATC401.0652002E-861.24444640
GACGAAT308.306593E-658.40692546
CGTTATA252.3915795E-455.9873628
ACGTTAT252.393176E-455.979827
AATCTCG2350.055.09394536
CGTCTTA2700.053.222448
CGACTAA200.006910071252.57334548
TACGCAC200.00693227952.53071249
AAATCCG200.00695825552.48106410
CACGATA200.00695825552.48106421
ACCGTTA200.00695825552.4810648
TATGACG5550.051.13169543
TATCGTA1650.050.90447238
CTCGAAT2000.050.745439
CGTATGA5800.050.72616241
ATCGTAT1600.050.30793839
TCTTTAT2400.049.565451
Produced by FastQC (version 0.11.5)