FastQCFastQC Report
Wed 25 May 2016
SRR1781244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267909
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7454527.824746462418208No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG28931.0798442754816002No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8480.3165253873516754No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7070.26389557648305956No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3960.14781138371611258No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3020.11272484313703535No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGT2770.10339331638728076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTAA150.002223037470.038
TTCATTT150.002223037470.022
CAAGTGT308.362635E-658.33333233
TTGTGCG553.6379788E-1157.2727242
CGTAACA252.3877172E-455.99999656
GCCGTAA252.3877172E-455.99999654
TTTAGGA200.006946248552.52
CAAGTTT200.006946248552.521
TTATGGG200.006946248552.52
TATATAA200.006946248552.540
AGAACTA200.006946248552.57
GCTCTAG200.006946248552.521
TGACTTA200.006946248552.540
AAGTGTA352.0831509E-550.034
AGTGTAG352.0831509E-550.035
CGTATCA5550.048.55855656
GTATCAT5500.048.36363657
TAGATCT5600.048.12539
GCCGTAT5600.048.12554
TCATTAA5550.047.92792560
Produced by FastQC (version 0.11.5)