FastQCFastQC Report
Wed 25 May 2016
SRR1781244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267909
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3695413.793489580417232No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG82663.0853760045388543No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT62792.3437062584683606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48991.8286059818819076No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC43141.6102482559376505No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT10860.40536152200933895TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC10670.39826956167952554No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT9390.35049214472078205No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6380.2381405626537369No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5890.2198507702242179TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT5530.20641337170457133No Hit
CTTATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT5130.191482928904964No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT3860.14407877301621072No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT3770.1407194233862991No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT3720.13885311803634817No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG3440.12840180807662305No Hit
CTTATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT3290.12280289202677029No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT3200.11944354239685863No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGA3150.11757723704690771No Hit
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT3100.11571093169695681No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTAAGCCGT2940.10973875457711388No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT2760.10302005531729057No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACG2700.10078048889734947No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCTA150.002212284170.0856147
TAACCGA207.928214E-569.98096511
TGCAATC301.0232179E-769.98096533
TCTTTAT1450.067.5678251
AATCTCC1750.065.982059
ATTATAC14300.064.84251
GTATTCT600.064.1971449
AGGGGGG43900.061.0540170
AGTCTAC308.3022405E-658.40467548
ATACAAA2200.057.2571534
CAAATCT1900.057.0897337
TATACAA2100.056.6512573
ACGTATT252.3733356E-456.0684947
AATATGC252.3821188E-456.026651
GCTTGAC252.3843187E-456.01613656
CAAGTAT252.3909278E-455.9847739
CTATACA1250.055.984771
TACAAAT2250.055.984775
GACTAAA252.3909278E-455.9847726
AATATAC950.055.248131
Produced by FastQC (version 0.11.5)