FastQCFastQC Report
Wed 25 May 2016
SRR1781243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387001
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7431019.201500771315835No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATCTCGGTG25220.6516779026410785No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8150.21059377107552693No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8010.20697620936380007No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4440.11472838571476561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG150.002223900569.999121
GCCGTAT4850.054.84465454
CGTATCA4750.053.78877656
GATTCGA200.00694892552.4993222
TCGGTGG5750.051.12977245
TCTCGGT5000.051.09934243
CCGTATC5100.050.78365755
CGGTGGT5400.050.554946
GTATCAT5250.049.99935557
TAGATCT5350.049.71898339
ATCTCGG5200.049.1339842
TGGTCGC5550.048.55793449
GTAGATC5650.047.69849838
CTCGGTG5800.047.67179544
TATCATT5450.047.5223258
CATTAAA5500.047.09029861
ATCATTA5450.046.88012759
CATTGCG305.8749184E-446.66606547
CGATTAG305.8749184E-446.66606552
GTGTAGA6450.046.66606536
Produced by FastQC (version 0.11.5)