FastQCFastQC Report
Wed 25 May 2016
SRR1781243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387001
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT356549.212896090707776TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG72391.8705378022279013TruSeq Adapter, Index 5 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT54341.404130738680262No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46031.1894026113627614No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC37410.9666641688264371RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT12030.31085190994338513No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC9940.2568468815326059RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT8540.22067126441533744TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT8420.21757049723385727No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT6170.15943111258110446No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT5700.14728644112030717No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC5610.14496086573419706TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT5010.12945702982679633No Hit
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT4720.12196350913821928TruSeq Adapter, Index 5 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT4490.11602037204038232No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4460.11524518024501229TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT4380.11317800212402553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG38900.061.4466670
ATTATAC13900.058.9305461
TAAGACG800.056.9211343
CCGTAAT1500.056.0526747
GCCGTAA1650.053.0801846
TCGAATG2450.052.85216540
TCGAATC200.006926322452.54258342
CGTTAGC200.006929866552.5357941
TCGCATA200.006944054752.5086358
ACGTGTA200.006944054752.508631
TTGTCTA200.00695115752.49505640
GTATCGT200.0069547152.48827727
AACTCGT1000.052.48827737
TGCCGTA3450.051.78779245
CGTCTTA4300.051.327348
CGTAATC1300.051.2019648
TCTTTAT2400.051.0500561
AAGCCGT3400.050.4886244
GTTATCT352.0876423E-549.98883434
AATGCCG1900.049.7771843
Produced by FastQC (version 0.11.5)