FastQCFastQC Report
Wed 25 May 2016
SRR1781242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136470
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6016744.0880779658533No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8160.5979336117828093No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.2989668058914047No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG3570.26159595515497913No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3170.23228548398915513No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2060.15094892650399355No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1590.11650912288415037No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.11577636110500476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATG150.002220693370.059
CTTGGGC150.002220693370.01
TTGTGCG308.340743E-658.3333322
TGTGCGC308.340743E-658.3333323
TGGGCAG252.3835506E-456.0000045
AAAAAGG252.3835506E-456.00000470
ACTGACT200.00693898152.549
TCTGGCT200.00693898152.566
CACAAAT200.00693898152.558
CTGTGCA200.00693898152.559
CTTGTGC200.00693898152.51
ACCTCAG200.00693898152.58
ATTAAAA751.4551915E-1146.66666862
TGTGGGT451.6748108E-646.66666837
TGTGGGC305.861105E-446.6666643
GCCGTAT901.8189894E-1242.7777854
CATTAAA757.4760464E-1042.061
TCATTAA855.638867E-1141.1764760
TTAAAAA855.638867E-1141.1764763
GTGTAGG350.001251883840.00000436