FastQCFastQC Report
Wed 25 May 2016
SRR1781242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136470
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3312024.269070125302264No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG61274.48963142082509No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC29732.178500769399868No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC11320.8294863339928189No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT7330.5371143841137246RNA PCR Primer, Index 48 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7230.5297867663222686No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT7190.5268557192056863No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT7110.5209936249725214No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6300.46163992086172784No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT5850.4286656408001759No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT5630.4125448816589727No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC5080.37224298380596466No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4990.3656481277936543No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT3870.28357880852934714No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTCGTCAATCTCGTATGCCGT3080.22569062797684475No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2610.19125082435700153RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT2510.18392320656554553No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2510.18392320656554553No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGACAATCTCGTATGCCGT2450.17952663589067194No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAG2330.17073349454092474No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2250.16487140030775996No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT2170.15900930607459515RNA PCR Primer, Index 48 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACG2040.14948340294570234No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2040.14948340294570234No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2000.14655235582911996No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGA1990.14581959404997435No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTAAATCTCGTATGCCGT1580.11577636110500476No Hit
CTTATACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1570.11504359932585917No Hit
CTTATACACATCTCCGAGACCACGAGACTTTCGTCAATCTCGTATGCCGT1570.11504359932585917No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCG1510.11064702865098555No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1410.10331941085952956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG252.8185332E-670.0377743
AAAGCCG252.8185332E-670.0377743
TCATATG252.8185332E-670.0377750
ACTGCTA301.0137228E-770.03776653
TTATGCC150.002215944570.03776643
CTTCAGA150.002215944570.03776651
TAAGCAG207.882535E-570.03776643
TAAGACG207.882535E-570.03776643
AGCAGTC207.882535E-570.03776645
CCGTAAT207.882535E-570.03776647
TAATCTG207.882535E-570.03776650
GTCTTAA150.002215944570.03776649
TACTGCA207.882535E-570.03776652
CTAAAGC207.882535E-570.03776651
CATATGC207.882535E-570.03776651
TAAAGCT150.002215944570.03776652
CTACTGA150.002215944570.03776651
GACTACT207.882535E-570.03776649
TCGTATC252.8246832E-670.012140
GATAGAA150.002219170670.0120956
Produced by FastQC (version 0.11.5)