FastQCFastQC Report
Wed 25 May 2016
SRR1781241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286680
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5483119.12620343239849No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7780.2713827263848193No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3940.13743546811776197No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG3050.10639040044649085No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG200.00694722152.4990815
GCGAATA200.00694722152.499082
TCTTACG200.00694722152.499084
TCGACAT200.00694722152.4990851
ATCGAGA200.00694722152.499081
GTAATCT350.001254300439.9993024
GCCGTAT701.9199433E-839.99930254
CCGTATC851.06043444E-732.940655
CAACGCT552.9881982E-431.81762520
TTGTGCG450.00430348831.1105692
ACCCGCA450.00430348831.11056946
TCTCGGT1202.2882887E-929.16615943
CGCGTCT604.975583E-429.1661597
CGTATCA1004.4058106E-727.99951256
GTTCAAA500.00720209827.9995121
GCGTGTG500.00720209827.9995128
CGCCGTA1004.4058106E-727.99951253
TAATGTC500.00720209827.9995124
GTCTAGG657.945497E-426.922611
TGGTCGC1207.370545E-826.24954249
Produced by FastQC (version 0.11.5)