FastQCFastQC Report
Wed 25 May 2016
SRR1781241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286680
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2902510.124529091670155No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG54631.905609041439933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC33311.1619227012697084No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC8310.28987023859355376No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7500.2616157388028464No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT6750.23545416492256172No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT6590.22987302916143434No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT6370.22219896748988419TruSeq Adapter, Index 2 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6350.22150132551974328No Hit
CTTATAAACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT5390.1880145109529789No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT4540.15836472722198966No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4360.15208594949072135No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT4180.14580717175945304No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT4170.1454583507743826No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT3690.1287149434910004No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT3480.12138970280452073No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT3390.11825031393888656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG26750.062.7821470
AAGCCGT1200.058.3947644
TAAGCCG1200.058.3947643
ATCGTAA308.363868E-658.33362639
TAAATCG308.381188E-658.31327436
CGTAAGC1350.057.09709541
TATCGTA1050.056.66694638
ATATCGT1050.056.65706337
GAACGTA1550.056.44204331
TAACCGA252.3899997E-455.99051311
TATATCG1000.055.98074736
TCGTAAG1350.054.44471440
ACGTAAC1300.053.8370333
TCGTATT200.00694660352.5002640
AACGTAT1400.052.49110432
AAATCGT407.473209E-752.49110437
CCGTATC200.006951398752.49110434
TCCGGAT200.006956196452.48194524
TACCCGT200.006956196452.4819456
CAACTAG200.006956196452.48194520
Produced by FastQC (version 0.11.5)