FastQCFastQC Report
Wed 25 May 2016
SRR1781240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65473
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3505653.54268171612726No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5550.8476776686573092No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3460.528462114153926No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG2140.32685229025705254No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120.3237975959555847No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.19397308814320405No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1070.16342614512852627No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG970.14815267362118736No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG920.1405159378675179No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690.10538695340063843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC252.8111517E-670.000013
CATCTGG150.00221551670.09
CTGCAGA150.00221551670.018
GTTGTGG150.00221551670.01
GTTGTGC150.00221551670.01
CATTAAA750.065.33333661
GTGTAGA900.062.22222536
TGTAGAT850.061.76470637
TCTCGGT800.061.2543
ATTAAAA800.061.2562
TCATTAA750.060.66666860
TAGATCT750.060.66666839
TTAAAAA750.060.66666863
TTGTGCG352.932502E-760.02
CGTATCA650.059.23076656
TATCATT650.059.23076658
GCCGTAT650.059.23076654
CCGTATC601.8189894E-1258.33333255
ATCATTA800.056.87559
CTTGTGC252.3743576E-456.0000041