FastQCFastQC Report
Wed 25 May 2016
SRR1781240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65473
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1679025.64415866082202No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG37765.76726284117117No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC13712.0939929436561635No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5600.8553144044109786No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5570.850732362958777No Hit
CTTATAAACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4940.7545094924625418No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4740.723962549447864RNA PCR Primer, Index 47 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4660.7117437722419928No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT3580.5467902799627328No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT3500.5345715027568616No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT3310.5055519068929177No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT3100.473477616727506No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3000.4582041452201671No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTAGATCTCGTATGCCGT2730.416965772150352No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT2460.37572739908053704No Hit
CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT2040.3115788187497136No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1770.27034044567989857No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1670.2550669741725597No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1490.22757472545934967No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAG1450.2214653368564141No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT1450.2214653368564141No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCG1440.2199379897056802No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACG1430.21841064255494633No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1360.20771921249980907No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1360.20771921249980907No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGGTAGATCTCGTATGCCGT1240.1893910466910024No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT1220.1863363523895346No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGA1210.18480900523880073No Hit
CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT1120.1710628808821957No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1020.1557894093748568No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG950.1450979793197196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCC830.1267698135109129No Hit
TCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT750.11455103630504178No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT730.111496342003574No Hit
CTTATACACATCTCCGCGCCCACGAGACAATGGTAGATCTCGTATGCCGT730.111496342003574No Hit
CTTATACACATCTCAGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT690.10538695340063843No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG680.10385960624990453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAC252.80112E-670.0420542
CGAATGA252.80112E-670.0420541
GCCGTAT252.80112E-670.0420546
CGTCATA150.00221023970.04204648
TGCACTC207.849549E-570.04204645
CGTCAAC207.849549E-570.04204648
CTGATAG207.849549E-570.04204654
CCGTATT207.849549E-570.04204647
CTGAATG150.00221023970.04204654
TGCTTTA150.00221023970.04204655
TGCTTCA150.00221023970.04204655
CACTCTT150.00221023970.04204647
GCAGACT207.849549E-570.04204646
CAGACTT150.00221023970.04204647
CAGATTG150.00221023970.04204654
CGAAAGC150.00221023970.04204641
ATGCAGA301.0050644E-770.04204644
ATGCACT207.849549E-570.04204644
CTGCTTT150.00221023970.04204654
CTGCTTC150.00221023970.04204654
Produced by FastQC (version 0.11.5)