FastQCFastQC Report
Wed 25 May 2016
SRR1781239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190379
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5508828.935964575924867No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7400.38869833332457887No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470.23479480404876588No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.17543951801406668No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG3130.16440888963593672No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2050.10767994369126846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA950.055.2631656
GCCGTAT900.054.44444354
TTAGGTT200.006943173752.544
ACTATCG200.006943173752.519
TGAAAGG200.006943173752.55
TCTACGA200.006943173752.550
CTAGTGG200.006943173752.54
GCGATAC200.006943173752.530
TGCTACG200.006943173752.57
GATCTAG200.006943173752.541
CCGTATC900.050.55555355
TTGTGCG305.8669806E-446.6666642
TGTGCGC451.6783342E-646.6666643
GTGTGGC404.580301E-543.7510
GTAGATC1150.042.608738
TAGATCT1150.042.608739
ATCTCGG1300.040.38461342
TCATTAA1150.039.5652260
ATCATTA1150.039.5652259
TCTCGGT1250.039.243
Produced by FastQC (version 0.11.5)