FastQCFastQC Report
Wed 25 May 2016
SRR1781239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190379
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2984415.676098729376664No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG52982.7828699593967823No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC27041.4203247206887315No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC8910.4680138040435132No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7470.39237520945062215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.3587580562982262No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT6530.3430000157580405Illumina PCR Primer Index 9 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6290.330393583325892No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6190.32514090314583016No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT5630.2957258941374836No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT5240.27524044143524234No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT4830.25370445269698866No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC4390.23059265990471636No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT4250.22323890765262974No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT3720.1953997026983018No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAAACAGATCTCGTATGCCGT3130.16440888963593672No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT2890.15180245720378824No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2760.1449739729697078No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2240.11766003603338603No Hit
CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT2220.11660949999737365No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2030.1066294076552561No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAG2020.1061041396372499No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2020.1061041396372499No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCG1920.10085145945718803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGT150.002221416270.0051
GAAATCG150.002223732969.9866136
CACGAAT150.002223732969.9866139
TACAGGT150.002223732969.986617
AGGGGGG29250.062.9281370
AATGACG401.0581061E-861.27046643
CGAATGC1150.060.873941
TCTTTAT2250.059.1153261
CTCGAAT950.058.93608539
TAAGACG308.336829E-658.35282543
TCGAATG1400.057.48899540
ATATCGA553.6379788E-1157.2617737
TTTATAC2350.056.5849153
CCGACAT252.3758331E-456.04817647
CGTAAGA252.385112E-456.00399441
TTGTGCG252.3882114E-455.989299
AGAAATC252.3882114E-455.9892935
AGTACAG252.3882114E-455.989295
TGCCGAC1450.055.56545
ATCGTAT1600.054.67703239
Produced by FastQC (version 0.11.5)