FastQCFastQC Report
Wed 25 May 2016
SRR1781238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223871
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3958617.682504656699617No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5240.23406336684965895No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2690.12015848412701957No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG2420.10809796713285777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT509.767973E-1055.99999654
TTGTACG200.006944763552.54
ATAGACT200.006944763552.54
CAAGCCG200.006944763552.521
TATACGC200.006944763552.53
TAATGTA200.006944763552.536
ATCGCTA200.006944763552.548
CGTATCA552.2719178E-950.90909656
CCGTATC604.9094524E-946.66666855
CCTATCC305.869206E-446.6666689
TACTTGA350.001253604140.01
CGCCGTA801.4151738E-939.37553
ATCTAGG400.002415288235.02
TGTGCGC400.002415288235.03
AGACCTA501.7060955E-435.05
GCTCGAA400.002415288235.02
TGCGTCA400.002415288235.031
TCTCGGT957.587005E-933.15789843
TGGTCGC957.587005E-933.15789849
CTATGTG450.004301124731.1111139