Basic Statistics
Measure | Value |
---|---|
Filename | SRR1781238_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 223871 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 19512 | 8.715733614447606 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG | 4375 | 1.954250438868813 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 1934 | 0.8638903654336649 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 710 | 0.3171469283649959 | No Hit |
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT | 582 | 0.2599711440963769 | RNA PCR Primer, Index 42 (95% over 22bp) |
CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 499 | 0.22289622148469435 | No Hit |
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 495 | 0.22110947822629998 | No Hit |
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 472 | 0.21083570449053252 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 448 | 0.20011524494016641 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT | 340 | 0.15187317696351918 | No Hit |
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 329 | 0.14695963300293471 | No Hit |
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC | 317 | 0.1415994032277517 | RNA PCR Primer, Index 32 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT | 300 | 0.13400574437957571 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT | 289 | 0.12909220041899128 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT | 243 | 0.10854465294745635 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGTAC | 15 | 0.002223655 | 69.991295 | 34 |
AAACGCG | 15 | 0.002223655 | 69.991295 | 69 |
CGTCTTA | 60 | 0.0 | 64.201706 | 48 |
ATCGATC | 60 | 0.0 | 64.15868 | 33 |
GCCGTAT | 30 | 8.331142E-6 | 58.36519 | 46 |
AGGGGGG | 1670 | 0.0 | 56.789337 | 70 |
TCGATAT | 185 | 0.0 | 56.74969 | 34 |
ATATCGT | 165 | 0.0 | 55.14465 | 37 |
CTCGATA | 180 | 0.0 | 54.43767 | 33 |
CAATCGA | 85 | 0.0 | 53.522747 | 31 |
TATCGTA | 170 | 0.0 | 53.522747 | 38 |
AATCGAT | 85 | 0.0 | 53.522747 | 32 |
TATGACG | 220 | 0.0 | 52.516926 | 43 |
AAGCGGG | 20 | 0.006948181 | 52.493465 | 70 |
TAAGCCG | 75 | 0.0 | 51.349884 | 43 |
TCGATCA | 55 | 2.2755557E-9 | 50.902756 | 34 |
CGTATGA | 255 | 0.0 | 50.789337 | 41 |
ATGACGA | 50 | 6.155824E-8 | 49.015797 | 44 |
ATCGTAT | 225 | 0.0 | 48.216225 | 39 |
GATATCG | 205 | 0.0 | 47.79893 | 36 |