FastQCFastQC Report
Wed 25 May 2016
SRR1781238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223871
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT195128.715733614447606No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG43751.954250438868813No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC19340.8638903654336649No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7100.3171469283649959No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5820.2599711440963769RNA PCR Primer, Index 42 (95% over 22bp)
CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4990.22289622148469435No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4950.22110947822629998No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4720.21083570449053252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4480.20011524494016641No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT3400.15187317696351918No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3290.14695963300293471No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3170.1415994032277517RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT3000.13400574437957571No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2890.12909220041899128No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT2430.10854465294745635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAC150.00222365569.99129534
AAACGCG150.00222365569.99129569
CGTCTTA600.064.20170648
ATCGATC600.064.1586833
GCCGTAT308.331142E-658.3651946
AGGGGGG16700.056.78933770
TCGATAT1850.056.7496934
ATATCGT1650.055.1446537
CTCGATA1800.054.4376733
CAATCGA850.053.52274731
TATCGTA1700.053.52274738
AATCGAT850.053.52274732
TATGACG2200.052.51692643
AAGCGGG200.00694818152.49346570
TAAGCCG750.051.34988443
TCGATCA552.2755557E-950.90275634
CGTATGA2550.050.78933741
ATGACGA506.155824E-849.01579744
ATCGTAT2250.048.21622539
GATATCG2050.047.7989336