FastQCFastQC Report
Wed 25 May 2016
SRR1781237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199142
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7809139.213726888351026No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8050.4042341645659881No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6770.3399584216287875No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG5980.300288236534734No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3730.1873035321529361No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2440.12252563497403862No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2200.11047393317331351No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120.10645669923973848No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTA150.002222196770.034
TGTAGGT308.354778E-658.33333237
TTGATAG252.3862226E-455.99999628
CTATAGG252.3862226E-455.9999964
TTGTGCG200.0069436452.52
ACATTTG200.0069436452.523
ACCGATA200.0069436452.58
TCTAGGA200.0069436452.52
TAGCCAG200.0069436452.55
CTGAAAT200.0069436452.521
TCCAGTG200.0069436452.559
AGCATTA200.0069436452.559
AATTGAC200.0069436452.551
GGTGCAA200.0069436452.528
TAAGGCG200.0069436452.543
ATACGCA200.0069436452.54
TATACGC200.0069436452.53
TTGGGCG200.0069436452.52
GCCGTAT1050.046.66666454
CGTATCA1150.045.65217656
Produced by FastQC (version 0.11.5)