FastQCFastQC Report
Wed 25 May 2016
SRR1781237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199142
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3987520.023400387663074No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG75483.7902602163280474No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC31021.5576824577437205No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC12660.6357272699882497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12100.6076066324532243No Hit
CTTATAAACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT11460.575468760984624No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT10240.5142059434976047RNA PCR Primer, Index 15 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT10130.508682246838939No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT9570.4805616093039138No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT8520.4278354139257414No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT8360.4198009460585914No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT6900.34648642677084696No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC6210.3118377840937622No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5940.2982796195679465No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT5760.2892408432174027No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT5530.2776912956583744No Hit
CTTATACACATCTCCGAGCCAACGAGACATAAGTTAATCTCGTATGCCGT3840.19282722881160178No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAGTTAATCTCGTATGCCGT3350.16822167096845467No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAG3150.15817858613451707No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3060.15365919795924515No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3010.15114842675076076No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3010.15114842675076076No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACG2780.13959887919173253No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGA2730.13708810798324814No Hit
CTTATACACATCTCCGAGACCACGAGACATAAGTTAATCTCGTATGCCGT2450.12302778921573551No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2430.12202348073234175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC150.00221483470.0585842
AATCTGC150.002221468170.00577551
ACATCAG150.002221468170.00577550
TAAAGGG150.002223682669.988269
GAACTAC150.002223682669.988230
TAACTCG150.002223682669.988236
CAAAGCG150.002223682669.988224
TCTTTAT2050.063.1600841
AACGTAT453.8380676E-1062.21172739
CTGATAG401.058288E-861.27044754
TAGACAT401.0626536E-861.23967424
TAACGTA401.0626536E-861.23967438
AGGGGGG36200.060.9013370
ACGTATG1050.060.0049540
AAATCGT352.9668627E-759.9898837
AATCGTA352.9668627E-759.9898838
CTTTATA2200.058.853712
CTTAAAC3600.057.3514371
TTTATAC2200.057.263073
GAATGCC1600.056.92259242
Produced by FastQC (version 0.11.5)